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Thermo Scientific™ Transcription Promoter Sequencing Primers
Perform sequencing of DNA fragments located downstream from the corresponding RNA polymerase promoter sequences in cloning vectors, such as pTZ19R, pTZ57R and pBluescript II.
Manufacturer: Thermo Scientific™ SO116
Description
Transcription Promoter Sequencing Primers are single-stranded oligonucleotides with 5'- and 3'-hydroxyl ends. The primers are complementary to the SP6, T3, or T7 RNA polymerase promoter regions respectively. Primers are supplied as 10 µM aqueous solutions.
The primers are complementary to the SP6 RNA polymerase promoter regions respectively. Primers are supplied as 10μM aqueous solutions.Recommended for:
Sequencing of DNA fragments located downstream from the corresponding RNA polymerase promoter sequences in common cloning vectors, such as pTZ19R, pTZ57R, pBluescript II.
Note:
Primers cannot be used for certain plasmids that contain truncated, but still fully functional promoters. Primers are not phosphorylated.
Specifications
| Sequencing of DNA fragments located downstream from the corresponding RNA polymerase promoter sequences in common cloning vectors, such as pTZ19R, pTZ57R, pBluescript II. | |
| SP6 promoter sequencing primer, 18-mer5'-d-3' | |
| 0.1 A260 units, 56µL (3.3µg) |