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Thermo Scientific™ RiboRuler High Range RNA Ladder

Catalog No. FERSM1821
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FERSM1821 5 x 20 μL
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Catalog No. FERSM1821 Supplier Thermo Scientific™ Supplier No. SM1821
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Description

Perform RNA sizing and quantification on native or denaturing gels with this mixture of 8 chromatography purified single-stranded RNA transcripts (200 to 6000 bp long).

RiboRuler High Range RNA Ladder 200 to 6000 bases is a mixture of 8 chromatography purified single-stranded RNA transcripts (200 to 6000 bp long), produced from specific templates that contain a fragment of the pTZ19R polylinker and Lambda phage fragments.

The RiboRuler High Range RNA Ladder is available in 1 mM EDTA, pH 6.0.

RiboRuler High Range RNA Ladder is composed of chromatography-purified transcripts that makes them free from NTPs and RNA degradation products. The concentration of each RNA transcript is determined spectrophotometrically (see photo for precise quantities). To minimize the possibility of contamination during the use, the ladder is aliquotted in smaller volumes.

RiboRuler High Range RNA Ladder is optimal for end-labeling using T4 Polynucleotide Kinase (see protocol in the Resources) and is ideal for Northern blots.

Highlights

  • Sharp bands of uniform intensity
  • Easy-to-remember band sizes and quantities
  • Predetermined concentration of ladder bands allows for approximate RNA quantification
  • Supplied with 2X RNA Loading Dye
  • Stable for 6 months at -20°C

Specifications

Concentration 2X
Gel Type Agarose
Label or Dye Xylene Cyanol FF, Bromophenol Blue, Ethidium Bromide
Size Range 200 to 6000 bp
Quantity 5 x 20 μL
Product Line RiboRuler
Product Type High Range DNA Ladder

Frequently Asked Questions (FAQs)

What is the recommended voltage for electrophoresis with Thermo Scientific DNA and RNA ladders? What does V/cm mean?

Each of Thermo Scientific DNA and RNA ladders has a recommended voltage given in V/cm. Please refer to the gel picture in the product information sheet. V/cm stands for the amount of voltage recommended per cm of the distance between the two electrodes of the gel box (not gel length).
What are the end functional groups of Thermo Scientific DNA and RNA ladders?

Thermo Scientific DNA and RNA ladders have 5′-phosphate and 3′-hydroxyl groups.
Can I use Thermo Scientific 2X RNA Loading Dye for native gel electrophoresis?

Thermo Scientific 2X RNA Loading Dye contains 95% formamide as a denaturing agent and hence cannot be used for native gel electrophoresis.
What kind of RNA molecular weight ladders do you offer?

We offer the following RNA Ladders:


RiboRuler High Range RNA Ladder (Cat. No. SM1821)
RiboRuler Low Range RNA Ladder (Cat. No. SM1831)
Century Marker Templates, 100-500 bp (Cat. No. AM7780)
Century-Plus Marker Templates, 0.1-1 kb (Cat. No. AM7782)
Decade Markers, 10-150 bp (Cat. No. AM7778)
Millennium Markers-Formamide, 0.5-9 kb (Cat. No. AM7151)
RNA 6000 Ladder, 0.2-6 kb (Cat. No. AM7152)
RNA Century-Plus Markers, 0.1-1 kb (Cat. No. AM7145)
RNA Millennium Markers, 0.5-9 kb (Cat. No. AM7150)

For Research Use Only. Not for use in diagnostic procedures.