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  6. Thermo Scientific™ pUC19 DNA/MspI (HpaII) Marker

Thermo Scientific™ pUC19 DNA/MspI (HpaII) Marker
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Catalog No. FERSM0222
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5 x 50 μg
50 μg
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FERSM0222 5 x 50 μg
FERSM0221 50 μg
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Catalog No. FERSM0222 Supplier Thermo Scientific™ Supplier No. SM0222

Description

Perform sizing and approximate quantification of small linear double-stranded DNA fragments in agarose and non-denaturing polyacrylamide gels.

pUC19 DNA/MspI (HpaII) Marker is recommended for sizing and approximate quantification of small linear double-stranded DNA fragments in agarose and non-denaturing polyacrylamide gels. pUC19 DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme, then purified and dissolved in storage buffer. The DNA fragments contain blunt or sticky ends, depending on the restriction enzyme used for the markers preparation.

pUC19 DNA/MspI (HpaII) Marker, ready-to-use, is premixed with 6X DNA Loading Dye for direct loading onto agarose and polyacrylamide gels.

pUC19 DNA is digested to completion with the appropriate Fermentas PureExtreme restriction enzyme, then purified and dissolved in storage buffer. The DNA fragments contain blunt or sticky ends, depending on the restriction enzyme used for the marker's preparation. pUC19 DNA/MspI (HpaII) Marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively it can be labeled radioactively or non-radioactively with Klenow Fragment or Klenow Fragment, exo- with the fill-in reaction.
  • Sizing and approximate quantification of small DNA fragments
  • Sharp bands
  • Supplied with loading dye for sample DNA

Specifications

Concentration 6X
Gel Type Agarose
Ready to Load No
Gel Compatibility Agarose Gels
Quantity 5 x 50 μg
Product Type DNA/MSPI (HPAII) Marker

Frequently Asked Questions (FAQs)

What is the recommended voltage for electrophoresis with Thermo Scientific DNA and RNA ladders? What does V/cm mean?

Each of Thermo Scientific DNA and RNA ladders has a recommended voltage given in V/cm. Please refer to the gel picture in the product information sheet. V/cm stands for the amount of voltage recommended per cm of the distance between the two electrodes of the gel box (not gel length).

For Research Use Only. Not for use in diagnostic procedures.