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Thermo Scientific™ NoLimits 2000 bp DNA Fragment
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Catalog No. FERSM1701
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10 μg
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FERSM1701 10 μg
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Catalog No. FERSM1701 Supplier Thermo Scientific™ Supplier No. SM1701
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Description

Optimize applications where a custom DNA size standard is required with NoLimits, a collection of 36 chromatography-purified individual DNA fragments ranging from 10 -20,000bp.

NoLimits is a collection of 36 highly-purified individual DNA fragments ranging from 10 to 20,000 bp. Unlike other vendors, where custom standards are produced by PCR, Thermo Scientific NoLimits DNA fragments are produced using specifically designed plasmids purified by patented technology.

Plasmid DNA is digested with Thermo Scientific Restriction Enzymes, and the individual DNA fragments are chromatography-purified from the digestion mixture. As a result, we offer exceptionally pure DNA fragments that are free of any truncated or degraded molecules. Our custom DNA fragments are the best choice for applications, ranging from electrophoresis (both gel and capillary) to HPLC and beyond.

Highlights

  • Chromatography-purified individual DNA fragments
  • Broad range: 10 to 20,000 bp
  • Sharp peaks during capillary electrophoresis, sharp bands during gel electrophoresis
  • Precise DNA concentration 

Specifications

Gel Type Agarose
Ready to Load No
Size Range 10 to 20000 bp
Gel Compatibility Agarose Gels
Quantity 10 μg
Product Line NoLimits
Product Type DNA Fragment

Frequently Asked Questions (FAQs)

Are the Thermo Scientific DNA ladders compatible with GelRed and GelGreen gel stains?

The GelRed or GelGreen nucleic acid dyes can be used with our DNA Ladders. Nucleic acid binding dyes can affect DNA migration during electrophoresis, therefore we highly recommend post-staining of gels. Please follow the gel staining protocol provided by the manufacturer of nucleic acid gel dyes.
Which Thermo Scientific DNA ladders are optimal for fast electrophoresis separation?

For fast sizing and quantification of double-stranded DNA, we recommend using FastRuler DNA Ladders. These ladders are easily resolved in a short separation distance after an 8-14 min run.
What is the recommended voltage for electrophoresis with Thermo Scientific DNA and RNA ladders? What does V/cm mean?

Each of Thermo Scientific DNA and RNA ladders has a recommended voltage given in V/cm. Please refer to the gel picture in the product information sheet. V/cm stands for the amount of voltage recommended per cm of the distance between the two electrodes of the gel box (not gel length).
What are the end functional groups of Thermo Scientific DNA and RNA ladders?

Thermo Scientific DNA and RNA ladders have 5′-phosphate and 3′-hydroxyl groups.

For Research Use Only. Not for use in diagnostic procedures.