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  6. Thermo Scientific™ GeneRuler Ultra Low Range DNA Ladder

Thermo Scientific™ GeneRuler Ultra Low Range DNA Ladder
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Catalog No. FERSM1212
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5 x 50 μg
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FERSM1211 50 μg
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Catalog No. FERSM1212 Supplier Thermo Scientific™ Supplier No. SM1212
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Description

Perform sizing and approximate quantification of small dsDNA fragments on agarose or polyacrylamide gels. Ideal for siRNA analysis.

GeneRuler Ultra Low Range DNA Ladder is recommended for sizing and approximate quantification of a wide range of double-stranded DNA on agarose or polyacrylamide gels. It is ideal for denaturing polyacrylamide gel electrophoresis. The ladder is a mixture of chromatography-purified individual DNA fragments.

GeneRuler Ultra Low Range DNA Ladder can be labeled radioactively with T4 Polynucleotide Kinase.

GeneRuler Ultra Low Range DNA Ladder is recommended for sizing and approximate quantification of a wide range of double-stranded DNA on agarose or polyacrylamide gels. It is ideal for denaturing polyacrylamide gel electrophoresis. The ladder is a mixture of chromatography-purified individual DNA fragments. GeneRuler Ultra Low Range DNA Ladder can be labeled radioactively with T4 Polynucleotide Kinase. The ladder is recommended for analysis of siRNA (Figure 1). The ladder is supplied with 6X DNA Loading Dye for sample DNA.
  • Ideal for both DNA sizing and approximate quantification
  • Ideal for denaturing polyacrylamide gel electrophoresis
  • Ideal for analysis of siRNA
  • Sharp bands
  • Bright reference bands (given in red)
  • Supplied with loading dye for sample DNA

Specifications

Concentration 6X
Gel Type Agarose
Label or Dye Xylene Cyanol FF, Bromophenol Blue, Orange G
Ready to Load No
Size Range 10 to 300 bp
Gel Compatibility Agarose Gels
Quantity 5 x 50 μg
Product Line GeneRuler
Product Type DNA Ladder

Frequently Asked Questions (FAQs)

Are the Thermo Scientific DNA ladders compatible with GelRed and GelGreen gel stains?

The GelRed or GelGreen nucleic acid dyes can be used with our DNA Ladders. Nucleic acid binding dyes can affect DNA migration during electrophoresis, therefore we highly recommend post-staining of gels. Please follow the gel staining protocol provided by the manufacturer of nucleic acid gel dyes.
Which Thermo Scientific DNA ladders are optimal for fast electrophoresis separation?

For fast sizing and quantification of double-stranded DNA, we recommend using FastRuler DNA Ladders. These ladders are easily resolved in a short separation distance after an 8-14 min run.
What is the recommended voltage for electrophoresis with Thermo Scientific DNA and RNA ladders? What does V/cm mean?

Each of Thermo Scientific DNA and RNA ladders has a recommended voltage given in V/cm. Please refer to the gel picture in the product information sheet. V/cm stands for the amount of voltage recommended per cm of the distance between the two electrodes of the gel box (not gel length).
What are the end functional groups of Thermo Scientific DNA and RNA ladders?

Thermo Scientific DNA and RNA ladders have 5′-phosphate and 3′-hydroxyl groups.

For Research Use Only. Not for use in diagnostic procedures.