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Thermo Scientific™ GeneRuler Express DNA Ladder, ready-to-use
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Catalog No. FERSM1553
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FERSM1553 50 μg
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Catalog No. FERSM1553 Supplier Thermo Scientific™ Supplier No. SM1553
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Description

Get fast separation (in 5 to 15 minutes at 23  V/cm) of double-stranded DNA fragments in agarose gels. Premixed with Loading Dye for direct loading onto gel.

GeneRuler Express DNA Ladder, ready-to-use, is designed for fast separation (in 5 to 15 minutes at 23 V/cm) of double-stranded DNA fragments in agarose gels (see Figure 1 and Table 1). The band pattern of express ladders is not affected by the composition of electrophoresis buffer, voltage, or gel percentage. The ladder is a mixture of chromatography-purified individual DNA fragments.

GeneRuler Express DNA Ladder, ready-to-use, is premixed with 6X DNA Loading Dye for direct loading onto agarose gels.

The ladder is supplied with 6X DNA Loading Dye for sample DNA.

Highlights

  • Ideal for both DNA sizing and approximate quantification 
  • Ideal for fast separation under a wide range of electrophoresis conditions 
  • Sharp bands 
  • Bright reference bands (given in red)
  • Can be directly loaded and are stable at room temperature for 6 months 
  • Supplied with loading dye for sample DNA 

Specifications

Concentration 6X
Gel Type Agarose
Label or Dye Xylene Cyanol FF, Bromophenol Blue, Orange G
Ready to Load Yes
Size Range 100 to 5000 bp
Gel Compatibility Agarose Gels
Quantity 50 μg
Product Line GeneRuler
Product Type DNA Ladder

Frequently Asked Questions (FAQs)

Are the Thermo Scientific DNA ladders compatible with GelRed and GelGreen gel stains?

The GelRed or GelGreen nucleic acid dyes can be used with our DNA Ladders. Nucleic acid binding dyes can affect DNA migration during electrophoresis, therefore we highly recommend post-staining of gels. Please follow the gel staining protocol provided by the manufacturer of nucleic acid gel dyes.
Which Thermo Scientific DNA ladders are optimal for fast electrophoresis separation?

For fast sizing and quantification of double-stranded DNA, we recommend using FastRuler DNA Ladders. These ladders are easily resolved in a short separation distance after an 8-14 min run.
What is the recommended voltage for electrophoresis with Thermo Scientific DNA and RNA ladders? What does V/cm mean?

Each of Thermo Scientific DNA and RNA ladders has a recommended voltage given in V/cm. Please refer to the gel picture in the product information sheet. V/cm stands for the amount of voltage recommended per cm of the distance between the two electrodes of the gel box (not gel length).
What are the end functional groups of Thermo Scientific DNA and RNA ladders?

Thermo Scientific DNA and RNA ladders have 5′-phosphate and 3′-hydroxyl groups.

For Research Use Only. Not for use in diagnostic procedures.