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Thermo Scientific™ Aminoallyl-dUTP Solution (50 mM)

Numéro de catalogue. FERR1101
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50 μL
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Numéro de catalogue. Quantity
FERR1101 50 μL
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Numéro de catalogue. FERR1101 Fournisseur Thermo Scientific™ Code fournisseur R1101
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Description

Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH.

Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH. Aminoallyl-dUTP can be enzymatically incorporated into DNA and the resulting amine-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten.

Applications
• Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis
• PCR
• Nick-translation
• Random-primed labeling
• Primer extension

Aminoallyl-dUTP can be enzymatically incorporated into DNA with:
• Reverse transcriptases
• Taq DNA polymerase (Cat. No. EP0072)
• phi29 DNA Polymerase (EP0091)
• Klenow Fragment (EP0051)
• Klenow Fragment, exo- (EP0421)
• DNA Polymerase I (EP0041)

Spécifications

Concentration 50 mM
Content And Storage Aminoallyl-dUTP Solution, 50 mM (50 μL)

Store at –20°C.
Form Liquid
Polymerase DNA Polymerase
Labeling Method Direct Labeling
Label or Dye Aminoallyl
Quantity 50 μL
Shipping Condition Dry Ice
For Use With (Application) Standard PCR, Gene Expression, Non-Radioactive Labeling

For Research Use Only. Not for use in diagnostic procedures.