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Thermo Scientific⢠EZ-Link⢠Sulfo-NHS-LC-Biotinylation Kit
Biotinylate protein amines (e.g., lysines) with this very popular mid-length (22.4A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.
Supplier: Thermo Scientific⢠LSG21435
Description
Thermo Scientific⢠EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
Highlights:
- Water-soluble analog of NHS-LC-Biotin
- Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate or phosphate buffers
- Negative charge of -SO3 group keeps biotinylation localized at the cell surface
- Creates an amide bond
Specifications
EZ-Link Sulfo-NHS-LC-Biotinylation Kit | |
10 reactions | |
1 to 10 mg | |
Water | |
Antibodies, Proteins |
Sulfo-NHS-LC-Biotinylation Kit | |
Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody ⢠EZ-Link Sulfo-NHS-LC-Biotin, 25 mg ⢠PBS Pack (makes 500 mL), 1 pack ⢠Zeba Spin Desalting Columns (7K MWCO), 5 mL, 10 columns ⢠HABA Solution, 1 mL ⢠Avidin, 10 mg Store biotin and avidin reagents at -20°C. Store remaining kit components at 4°C. |
|
EZ-Link⢠| |
Biotin |
User Guide: EZ-Link Sulfo-NHS-LC-Biotinylation Kit
Version: B
File size: 357.8 kB
For Research Use Only. Not for use in diagnostic procedures.