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MilliporeSigma™ EZ-ChIP™
Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists
Supplier: MilliporeSigma™ 17371
Includes:
- DNA Purification Spin Columns and Collection Tubes
- ChIP Blocked Protein G Agarose
- Anti-RNA Polymerase II
- Control PCR Primers
- Normal Mouse IgG
- Bind, Wash and Elution Reagents
- Protease Inhibitor Cocktail II
- RNase A
- Proteinase K
- All required buffers
- ChIP Dilution Buffer
- Low Salt Immune Complex Wash Buffer
- High Salt Immune Complex Wash Buffer
- LiCl Immune Complex Wash Buffer
- TE Buffer
- 0.5M EDTA
- 5M NaCl
- SDS Lysis Buffer
- 1M Tris-HCl, pH 6.5
- 10X PBS
- 10X Glycine
- 1M NaHCO3
- Control Primers
- 20% SDS
- Spin Filters
- Collection Tubes
- Bind Reagent A
- Wash Reagent B
- Elution Reagent C
Description
- Easier: Spin columns make DNA purification easier and more reliable - no more messy phenol-chloroform extractions
- Quicker: All reagents to process your samples are included - you don't have to spend valuable time making them
- Greater Reproducibility: Positive and negative control antibodies and PCR primers are included to help validate your results and to troubleshoot your experiments
Specifications
| Store components at the temperatures indicated on the labels | |
| 22 individual chromatin immunoprecipitation (ChIP) reactions using inexpensive protein G agarose beads | |
| 22 Assays | |
| Stable for 1 year from date of shipment when stored as directed |