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MilliporeSigma™ EZ-ChIP™

Catalog No. 17371MI
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22 Assays
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Catalog No. Quantity
17371MI 22 Assays
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Catalog No. 17371MI Supplier MilliporeSigma™ Supplier No. 17371
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Includes

  • DNA Purification Spin Columns and Collection Tubes
  • ChIP Blocked Protein G Agarose
  • Anti-RNA Polymerase II
  • Control PCR Primers
  • Normal Mouse IgG
  • Bind, Wash and Elution Reagents
  • Protease Inhibitor Cocktail II
  • RNase A
  • Proteinase K
  • All required buffers
  • ChIP Dilution Buffer
  • Low Salt Immune Complex Wash Buffer
  • High Salt Immune Complex Wash Buffer
  • LiCl Immune Complex Wash Buffer
  • TE Buffer
  • 0.5M EDTA
  • 5M NaCl
  • SDS Lysis Buffer
  • 1M Tris-HCl, pH 6.5
  • 10X PBS
  • 10X Glycine
  • 1M NaHCO3
  • Control Primers
  • 20% SDS
  • Spin Filters
  • Collection Tubes
  • Bind Reagent A
  • Wash Reagent B
  • Elution Reagent C

Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists

  • Easier: Spin columns make DNA purification easier and more reliable - no more messy phenol-chloroform extractions
  • Quicker: All reagents to process your samples are included - you don't have to spend valuable time making them
  • Greater Reproducibility: Positive and negative control antibodies and PCR primers are included to help validate your results and to troubleshoot your experiments

Specifications

Content And Storage Store components at the temperatures indicated on the labels
For Use With (Application) 22 individual chromatin immunoprecipitation (ChIP) reactions using inexpensive protein G agarose beads
Quantity 22 Assays
Stability Stable for 1 year from date of shipment when stored as directed