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Gibco™ Expi293F™ Inducible GnTI- Cells
Expi293F Inducible GnTI- cell line is derived from engineered Expi293F cells that do not have N-acetylglucosaminyltransferase I (GnTI) activity, therefore lacking complex N-glycans, and stably express the tetracycline repressor (TetR) protein, thus enabling tetracycline-regulated protein expression
Supplier: Gibco™ A39242
Description
Cell line established by the selective knockout of the GnTI gene and stable integration of the regulatory plasmid pcDNA6/TR, which encodes the TetR gene under the control of the human CMV promoter. It is a powerful tool for tetracycline-inducible high-yield expression of homogeneously glycosylated recombinant proteins.
Features:
- Suspension, high-density culture in Expi293 Expression Medium
- Homogeneous N-glycosylation of expressed proteins
- Tetracycline-inducible protein expression with extremely low basal expression levels
- Equivalent protein yields to parental Expi293F cells—up to 1 g/L
For inducible expression of aprotein of interest, pcDNA5/TO mammalian expresson vector or an equivalent inducible vector is required for tetracycline-regulated gene expression.
Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in Expi293 Expression Medium and 10% DMSO. The cells should be thawed directly into Expi293 Expression Medium.
Specifications
| 1 vial Expi293F Inducible GnTI- Cells (1x107 cells/vial), store in vapor-phase liquid nitrogen |
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| Expi293 | |
| 1 vial | |
| Dry Ice |
For Research Use Only. Not for use in diagnostic procedures.