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Gibco™ Expi293F™ Inducible Cells DFS Item

Expi293F Inducible cell line is derived from engineered Expi293F cells that stably express the tetracycline repressor (TetR) protein, thus enabling tetracycline-regulated protein expression

Supplier:  Gibco™ A39241

Catalog No. A39241


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Description

Description

Cell line established by the stable integration of the regulatory plasmid pcDNA6/TR, which encodes the TetR gene under the control of the human CMV promoter. It is a powerful tool for tetracycline-inducible high-yield expression of recombinant proteins.

Features:

  • Suspension, high-density culture in Expi293 Expression Medium
  • Tetracycline-inducible protein expression with extremely low basal expression levels
  • Equivalent protein yields to parental Expi293F cells—up to 1 g/L
  • Significantly higher protein yields than other HEK293 Tet-inducible cell line alternatives

For inducible expression of a protein of interest, pcDNA5/TO mammalian expression vector or an equivalent inducible vector is required for tetracycline-regulated gene expression.

Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in Expi293 Expression Medium and 10% DMSO. The cells should be thawed directly into Expi293 Expression Medium

Specifications

Specifications

1 vial Expi293F Inducible Cells (1x107 cells/vial), store in vapor-phase liquid nitrogen

Expi293™
1 vial
Dry Ice
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For Research Use Only. Not for use in diagnostic procedures.