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Gibco™ Expi293™ Expression Medium

Catalog No. p-7075864
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A1435103 10 L
A1435101 1 L
A1435102 6 x 1 L
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Description

Expi293 Expression Medium designed for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells.

Expi293 Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density culture of Expi293F cells in the Expi293 Expression System for scalable transient protein expression.

  • Supports growth of suspension 293 cultures to densities of over 1.5 x 10^7 cells/mL
  • Transfection compatible serum-free suspension culture medium enables transfection efficiencies of greater than 70% using ExpiFectamine transfection reagent
  • Enables sustained, high level expression of high density transiently transfected cultures, achieving yields of up to 1 gram per liter of recombinant protein
  • Supports growth and transfection of 293 cells in culture formats of less than 1 mL in multiwell plates to greater than 10 L in disposable bioreactors
  • Does not contain phenol red

Expi293 Expression Medium is formulated with GlutaMAX-I reagent. It is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. Expi293 Expression Medium contains no human or animal-origin components. The medium is not recommended for adherent cell cultures.

Specifications

Cell Line Expi293F™
Classification Animal Origin-free, Chemically-defined, Protein-free, Serum-free
Form Liquid
Product Type Expression Medium
With Additives GlutaMAX
Without Additives No Phenol Red
Product Line Expi293
Quantity 10 L
Content And Storage One 10L Universal Bag
Store in the dark at 2-8°C.

For Research use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.