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Gibco™ Expi293™ Expression Medium
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Catalog No. a1435101
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Description

Expi293 Expression Medium designed for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells.

Expi293 Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density culture of Expi293F cells in the Expi293 Expression System for scalable transient protein expression.

  • Supports growth of suspension 293 cultures to densities of over 1.5 x 10^7 cells/mL
  • Transfection compatible serum-free suspension culture medium enables transfection efficiencies of greater than 70% using ExpiFectamine transfection reagent
  • Enables sustained, high level expression of high density transiently transfected cultures, achieving yields of up to 1 gram per liter of recombinant protein
  • Supports growth and transfection of 293 cells in culture formats of less than 1 mL in multiwell plates to greater than 10 L in disposable bioreactors
  • Does not contain phenol red

Expi293 Expression Medium is formulated with GlutaMAX-I reagent. It is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. Expi293 Expression Medium contains no human or animal-origin components. The medium is not recommended for adherent cell cultures.

Specifications

Cell Line 293E (HEK), 293 (HEK), Expi293F™, FreeStyle™ 293-F Cells, 293F
Cell Type Embryonic Kidney Cells
Classification Animal Origin-free, Chemically-defined, Protein-free, Serum-free
Concentration 1 X
Form Liquid
Product Type Expression Medium
Serum Level Serum-free
Sterility Sterile
Sterilization Method Sterile-filtered
With Additives High Glucose, GlutaMAX, Sodium Pyruvate, Sodium Bicarbonate, Dextrose, Pyridoxine HCl
Without Additives No HEPES, No Phenol Red, No Hypoxanthine, No Thymidine, No Insulin, No Vitamin A, No 2-Mercaptoethanol
Product Line Expi293
Quantity 1 L
Shipping Condition Room Temperature
Endotoxin Level < 1 EU/mL
Format Bottle(s)
Included Antibiotics No antibiotics
Inorganic Salts Calcium, Magnesium, Sodium Phosphate
Osmolality 275-295 mOsm/kg
Purity or Quality Grade Cell Culture Grade
Content And Storage Store in the dark at +2°C to +8°C
Culture Environment CO2
Culture Type Suspension Cell Culture
Expression System Expi293F™
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For Research use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.