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Gibco™ Essential 8™ Adaptation Kit

Catalog No. A25935
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Catalog No. A25935 Supplier Gibco™ Supplier No. A25935
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Description

Essential 8 Adaptation Kit

The Essential 8™ Adaptation Kit includes Essential 8™ Medium and recombinant human Laminin 521 (rhLaminin-521) for optimal survival during transition from feeder-dependent to feeder-free culture. rhLaminin-521 has been proven to promote cellular survival under stressful conditions in the absence of small molecule inhibitors. In addition, rhLaminin-521 has been shown to support PSC growth for >20 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages. rhLaminin-521 can also be purchased separately.

The Essential 8 Adaptation Kit is:
  • Robust–transition cells between feeder-dependent and feeder-free culture systems with minimal effort
  • Physiologically relevant–expand cells with minimal differentiation
  • Scalable–passage stem cell lines as single cells with no impact to genetic stability

    Trouble-free transition
    When used with Essential 8 Medium, rhLaminin-521 provides a robust culture matrix that supports pluripotent stem cell adherence and expansion during the transition from feeder-dependent culture systems.

    Confident expansion
    rhLaminin-521 is expressed in blastocysts and therefore provides an environment mimicking the human stem cell niche.

    Easy scale-up
    rhLaminin-521 promotes stable pluripotent stem cell maintenance as single cells in the absence of ROCK inhibitors.

    • Specifications

    Cell Type Pluripotent Stem Cells
    Classification Xeno-free
    Product Type Stem Cell Adaptation Kit
    Product Line Essential 8
    Quantity 1 Kit
    Shipping Condition Supplement and matrix - dry ice, Basal - ambient
    Content And Storage • 100 μg rhLaminin-521, store at -10 to -30°C, protect from light
    • 500 mL basal medium, store at 2-8°C, protect from light
    • 10 mL supplement, store at -5 to -20°C, protect from light

    For Research Use Only. Not for use in diagnostic procedures.