Stemcell Technologies EpiCult™-B Mouse Medium Kit, Size: 500 mL 

EpiCult™-B (mouse) is a serum-free liquid culture medium optimized for the culture of mouse mammary luminal and myoepithelial cells. It is ideal for the culture and evaluation of mouse mammary epithelial progenitors in the mammary colony-forming unit assay when used in conjunction with an irradiated feeder layer such as NIH 3T3 cells. This medium is also used for enzymatic dissociation of mouse mammary tissue when supplemented with collagenase and hyaluronidase. Addition of Human Recombinant EGF (Catalog #78006), Human Recombinant bFGF (Catalog #78003), and Heparin Solution (Catalog #07980) is required for culturing cells.