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MilliporeSigma™ PKA, Catalytic Subunit, Bovine Heart, Protein

Catalog No. 53957625UG
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Quantity:
25 μg
Catalog No. Quantity
53957625UG 25 μg
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Catalog No. 53957625UG

Supplier: MilliporeSigma™ 53957625UG

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Description

Native PKA catalytic subunit purified from bovine heart. The catalytic subunit of PKA dissociates from the PKA holoenzyme following binding of cAMP to the PKA regulatory subunits and phosphorylates target proteins at serine and/or threonine within the recognition motif, Arg-Arg-X-Ser-X. The purified monomeric catalytic subunit does not require cAMP for activation and is suitable for studies of PKA phosphorylation in vitro with purified substrates or following its introduction into intact cells or cell extracts.

Specifications

Concentration Concentration on Label
Formulation In 350mM K2HPO4, 100μM DTT, pH 6.8.
Purification Method SDS-PAGE
Quantity 25 μg
Immunogen PKA, Catalytic Subunit
Storage Requirements Following initial thaw, aliquot and freeze (−70°C).
Regulatory Status RUO
Species Bovine
Recombinant Native
Form Liquid
Purity or Quality Grade ≥95%
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