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Edvotekā„¢ Mapping of Restriction Sites on Plasmid DNA DFS Item

Perform electrophoresis of plasmid DNA in parallel with standard DNA markers.

Supplier:  Edvotekā„¢ EDV105

Catalog No. S10105


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Includes: Instructions, Ready-to-Load QuickStripā„¢ DNA Samples, UltraSpec-Agaroseā„¢, Electrophoresis Buffer (50X), 10X Gel Loading Solution, FlashBlueā„¢ DNA Stain, InstaStainā„¢ Blue Cards, & Disposable Pipets.

Requires :
  • Horizontal electrophoresis apparatus
  • DC power supply
  • Staining tray and net
  • Visualization system (white light)
  • Automatic micropipets with tips to obtain sample for five gels
  • Pipet pump
  • 250mL Flasks
  • Distilled or deionized water
  • Water bath (65°C)
  • Hotplate, Bunsen burner or microwave
  • Balance


Description

Description

  • In this experiment, plasmid DNA is mapped after the electrophoresis of plasmid DNA fragments in parallel with standard DNA markers
  • Teach students that DNA mapping is a common procedure used to determine the location of genes
  • Also show that genes can be excised if students know the location of specific restriction sites within DNA
Greener Choice Claims
  • Environmental benefits include:
    • Renewable Energy
Specifications

Specifications

Electrophoresis Kit
Electrophoresis of plasmid DNA in parallel with standard DNA markers
5 Gels (4 Lanes Each)
9 to Undergraduate
Instructions, Ready-to-Load QuickStripā„¢ DNA Samples, UltraSpec-Agaroseā„¢, Electrophoresis Buffer (50X), 10X Gel Loading Solution, FlashBlueā„¢ DNA Stain, InstaStainā„¢ Blue Cards, and Disposable Pipets
8 Lab Groups
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