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  5. Invitrogen™ Dynabeads™ CD15

Invitrogen™ Dynabeads™ CD15

Numéro de catalogue. 11138D
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11138D 2 mL
11137D 5 mL
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Numéro de catalogue. 11138D Fournisseur Invitrogen™ Code fournisseur 11138D
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Description

Dynabeads CD15 are superparamagnetic beads covalently coupled with an anti-human CD15 antibody that efficiently depletes human CD15+ myeloid cells directly from whole blood, buffy coat, or PBMC samples. Dynabeads CD15 can also be used to positively isolate CD15+ cells for molecular applications.

Dynabeads CD15 are superparamagnetic beads covalently coupled with an anti-human CD15 antibody that efficiently depletes human CD15+ myeloid cells (predominantly neutrophil and eosinophil granulocytes) directly from whole blood, buffy coat, or peripheral blood mononuclear cell (PBMC) samples. Dynabeads CD15 can also be used to positively isolate CD15+ cells for molecular applications. Advantages of Dynabeads CD15:

  • Efficient depletion of granulocytes (neutrophils and eosinophils)
  • Isolation of granulocytes directly from whole blood for molecular applications
  • No columns required

About Dynabeads CD15

Dynabeads CD15 are uniform, superparamagnetic polystyrene beads (4.5 μm diameter) coated with a monoclonal mouse IgM antibody recognizing the human CD15 membrane antigen. The CD15 antigen is predominantly expressed on human neutrophil and eosinophil granulocytes, and to a varying degree on monocytes.

Magnetic bead-based separation offers easy handling

Dynabeads CD15 is added to the sample under continuous mixing to optimize the binding of the Dynabeads to the target cells. By placing the sample on a magnet, the bead-bound target cells are separated from the rest of the sample in just 1 to 2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2 to 3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, PBMC, or buffy coat.

Automation-ready for use with KingFisher purification systems

The product is ideal for high-throughput workflows using KingFisher systems. A user guide and scripts for KingFisher Flex and Apex workflows are available for download on this page.

Learn more about Dynabeads products

Spécifications

Cell Type Granulocytes
Content And Storage This Product Contains: 2 mL Dynabeads™ CD15 coated with anti-CD15 monoclonal antibody.
Storage Conditions: 2°C to 8°C. Do not freeze.
Color Brown
For Use With (Application) Cell Depletion, Positive Isolation for Molecular Applications
Material Magnetic Polystyrene
High-throughput Compatibility Yes
No. of Cells Processes ∼8x 10^8 cells total
Output Viability >95%
Product Line Dynabeads
Purity or Quality Grade Research Grade
Quantity 2 mL
Sample Type PBMC, Blood
Shipping Condition Room Temperature
Starting Material Cell No. 1 x 10^7 PBMCs per isolation
Target Species Human
Diameter (Metric) 4.5 μm
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For Research Use Only. Not for use in diagnostic procedures.