Generate functional, human-specific sensory neurons in a dish with this easy-to-use culture system. After obtaining pluripotent stem cell (PSC)-derived neural crest cells (NCCs) with STEMdiff™ Neural Crest Differentiation Kit (https://www.stemcell.com/products/stemdiff-neural-crest-differentiation-kit.html), use the serum-free STEMdiff™ Sensory Neuron Differentiation Kit to generate sensory neuron precursors. STEMdiff™ Sensory Neuron Maturation Kit can then be used to maintain and mature the sensory neuron precursors. The resulting cells are BRN3A-positive and more than 70% TUJ1-positive. With the physiological glucose and osmolarity conditions provided by BrainPhys™ (https://www.stemcell.com/products/brainphys-neuronal-medium.html) as the basal medium, the neurons exhibit activity in response to sensory ligands and changes in temperature. The generated population of functional human sensory neurons can be used for drug discovery and pain research applications.
