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Gibco™ Defined Keratinocyte Serum-Free Medium (SFM) 
Defined Keratinocyte Serum-Free Medium (SFM) consistis of Defined Keratinocyte Serum Free Basal Medium that, with the addition of Defined Keratinocyte SFM Growth Supplement, eliminates the need for bovine pituitary extract, offering a defined media solution for the culture of keratinocytes.
Supplier: Gibco™ 10744019
Description
Defined Keratinocyte SFM is a serum-free defined medium optimized for the isolation and expansion of human keratinocytes without the need for bovine pituitary extract (BPE) supplementation or the use of fibroblast feeder layers.
The growth-promoting activity of BPE has been replaced by the inclusion of growth promoting agents which enhance consistent product performance. These growth-promoting agents also maintain cell morphology, physiological markers, and growth. The medium is formulated to inhibit fibroblast proliferation and has a low calcium concentration (<0.1 mM), allowing for the isolation and culture of highly enriched populations of undifferentiated keratinocytes.
Note: This formulation contains some human and animal origin components.
Note: A Defined Keratinocyte-SFM Master File has been submitted to the FDA. Permission to cross-reference the Master File may be obtained by contacting Technical Services or your local Sales Representative. Defined Keratinocyte-SFM consists of a basal liquid medium and frozen supplement which is added at the time of use. The fully supplemented medium has a shelf-life of 10 weeks under normal use conditions. Product performance is confirmed by evaluation of human primary keratinocyte growth.
Specifications
| Keratinocyte Cells, Epithelial Cells | |
| 1 X | |
| Kit | |
| Sterile-filtered | |
| Glutamine, Phenol Red | |
| • 500 mL Defined Keratinocyte-SFM Basal Medium; store in refrigerator (2-8°C) and protect from light • 1 mL Defined Keratinocyte-SFM Growth Supplement; store in freezer (-5°C to -20°C) and protect from light Complete Medium: store in refrigerator (2-8°C) and protect from light. |
| Serum-free | |
| Liquid | |
| Serum-free | |
| Sterile-filtered | |
| 500 mL | |
| Mammalian Cell Culture |
For Research Use Only. Not for use in diagnostic procedures.