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Gibco™ Defined Keratinocyte Serum-Free Medium (SFM)
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Catalog No. 10744019
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10744019 500 mL
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Catalog No. 10744019 Supplier Gibco™ Supplier No. 10744019
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Description

Defined Keratinocyte Serum-Free Medium (SFM) consistis of Defined Keratinocyte Serum Free Basal Medium that, with the addition of Defined Keratinocyte SFM Growth Supplement, eliminates the need for bovine pituitary extract, offering a defined media solution for the culture of keratinocytes.

Defined Keratinocyte SFM is a serum-free defined medium optimized for the isolation and expansion of human keratinocytes without the need for bovine pituitary extract (BPE) supplementation or the use of fibroblast feeder layers.

The growth-promoting activity of BPE has been replaced by the inclusion of growth promoting agents which enhance consistent product performance. These growth-promoting agents also maintain cell morphology, physiological markers, and growth. The medium is formulated to inhibit fibroblast proliferation and has a low calcium concentration (<0.1 mM), allowing for the isolation and culture of highly enriched populations of undifferentiated keratinocytes.

Note: This formulation contains some human and animal origin components.

Note: A Defined Keratinocyte-SFM Master File has been submitted to the FDA. Permission to cross-reference the Master File may be obtained by contacting Technical Services or your local Sales Representative. Defined Keratinocyte-SFM consists of a basal liquid medium and frozen supplement which is added at the time of use. The fully supplemented medium has a shelf-life of 10 weeks under normal use conditions. Product performance is confirmed by evaluation of human primary keratinocyte growth.

Specifications

Cell Type Keratinocyte Cells, Epithelial Cells
Classification Serum-free
Concentration 1 X
Form Liquid
Product Type Kit
Serum Level Serum-free
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives Glutamine, Phenol Red
Quantity 500 mL
Content And Storage • 500 mL Defined Keratinocyte-SFM Basal Medium; store in refrigerator (2-8°C) and protect from light
• 1 mL Defined Keratinocyte-SFM Growth Supplement; store in freezer (-5°C to -20°C) and protect from light

Complete Medium: store in refrigerator (2-8°C) and protect from light.
Culture Type Mammalian Cell Culture
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Frequently Asked Questions (FAQs)

Are the Defined Keratinocyte SFM basal medium and supplement available separately?

The Defined Keratinocyte SFM basal medium and supplement are not available separately. The kit can be ordered with catalog #10744-019.
Are the Keratinocyte SFM basal medium and supplement kit available separately?

The basal medium is not available for purchase individually, and can only be ordered as part of the Keratinocyte SFM kit (catalog # 17005-042). The supplements are available separately: Bovine Pituitary Extract is catalog # 13028-014 and the EGF is catalog # PHG0311.

Which serum-free media can be used to culture mouse keratinocytes?

The Keratinocyte SFM without calcium chloride (Cat. No. 37010-022) is appropriate for the culture of mouse keratinocytes. The medium should be supplemented with calcium chloride at a final concentration of 0.02 mM.
Which serum-free media can be used to culture human keratinocytes?

There are a variety of options for serum-free culture of human keratinocytes. Keratinocyte SFM (Cat. No. 17005-042) is a widely used kit that includes the basal medium, bovine pituitary extract (BPE) and Epidermal Growth Factor (EGF). If you would like a serum-free, defined culture system, the options are the Defined Keratinocyte SFM kit (Cat. No. 10744-019) or Epilife medium (Cat. No. M-EPI-500-CA) supplemented with EDGS (Cat. No. S-012-5). If you would like an animal origin free defined system, Epilife medium (Cat. No. M-EPI-500-CA) with S7 supplement (Cat. No. S-017-5) is an excellent choice.
For how long can I store Keratinocyte-SFM at 4 degrees C?

The base Keratinocyte-SFM has a shelf life of 12 months when stored at 2 to 8 degrees C, protected from light. Keratinocyte-SFM Supplement has a shelf life of 18 months when stored at -20 to 5 degrees C. The complete media should not be stored for longer than 4 weeks at 4 degrees C

For Research Use Only. Not for use in diagnostic procedures.