missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Cell Culture Media, Supplements, and Reagents
  4. Cell Culture Media
  5. Cell Target Type Cell Culture Media
  6. Stem Cell Culture Media
  7. Gibco™ StemPro™ MSC SFM

Gibco™ StemPro™ MSC SFM

Catalog No. A1033201
Encompass
Change view
Click to view available options
With Additives:
Low Glucose, Sodium Pyruvate, Sodium Bicarbonate, Adenosine, Deoxyribonucleosides
Without Additives:
No Glutamine, No Phenol Red
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. With Additives Without Additives
A1033201 Low Glucose, Sodium Pyruvate, Sodium Bicarbonate, Adenosine, Deoxyribonucleosides No Glutamine, No Phenol Red
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. A1033201 Supplier Gibco™ Supplier No. A1033201
Only null left

Description

StemPro™ MSC SFM is a serum-free medium (SFM) specially formulated for the growth and expansion of human mesenchymal stem cells (MSCs).

StemPro™ MSC SFM is a serum-free medium (SFM) specially formulated for the growth and expansion of human mesenchymal stem cells (MSCs). StemPro™ MSC SFM enables superior human MSC growth and increased consistency compared to classical serum-supplemented medium (DMEM + 10% FBS).

Using StemPro™ MSC SFM, human MSCs can be expanded beyond 5 passages while still maintaining their tri-lineage mesoderm differentiation potential (i.e., ability to differentiate into osteogenic, chondrogenic and adipogenic lineages). Each lot of StemPro™ MSC SFM is qualified using a human MSC performance assay and human MSCs growing in serum-supplemented medium can be transitioned directly into StemPro™ MSC SFM with little or no adaptation required. In addition, StemPro™ MSC SFM facilitates expansion of MSCs directly from primary human bone marrow.

Specifications

Cell Type Mesenchymal Stem Cells
Classification Serum-free
Form Liquid
Product Type Stem Cell Serum Free Media (SFM)
Serum Level Serum-free
With Additives Low Glucose, Sodium Pyruvate, Sodium Bicarbonate, Adenosine, Deoxyribonucleosides
Without Additives No Glutamine, No Phenol Red
Product Line StemPro
Quantity 1 Kit
Endotoxin Level Low (≤10 EU/mL)
Inorganic Salts Calcium, Sodium Phosphate
Purity or Quality Grade Research Grade
Species Human
Content And Storage This kit is shipped in two parts with separate storage requirements. These components are not sold individually:
• StemPro™ MSC SFM Basal Medium, 500 mL (ships at room temperature, store in the dark at 2°C to 8°C)
• StemPro™ MSC SFM Supplement, 75 mL (ships on dry ice, store in the dark at -5°C to -20°C)
Culture Environment CO2
Culture Type Adherent Cell Culture
Show More Show Less

Frequently Asked Questions (FAQs)

Can StemPro MSC SFM (Cat. No. A1003201) be used to isolate primary cells?

Yes. StemPro MSC SFM (Cat. No. A003201) can be used for both isolating primary cells and transitioning cells from serum-containing medium.

Do StemPro MSC SFM Basal Medium CTS and StemPro MSC SFM Supplement CTS contain L-Glutamine?

Both CTS StemPro MSC SFM and StemPro MSC SFM Supplement (Cat. No. A1033201) do not contain L-Glutamine.

What kinds of growth factors are used in mesenchymal stem cell (MSC) research?

Growth factors used for MSC maintenance and self-renewal: LIF, FGF-basic, EGF, HGF, PDGF, and Wnt3.

Growth factors or cytokines for MSC differentiation: BMP-2and TGF-β1.

(Arthritis Res Ther 9:204 (2007)

View our Gibco™ MSC research products.
How can I culture mesenchymal stem cells (MSCs) in vitro?

MSCs may be cultured in either serum-containing medium or serum-free medium. The standard culturing conditions are DMEM, low glucose with 10% FBS. MSCs can also be grown in reduced-serum (2%) Gibco MesenPRO RS Medium (Cat. No. 12746-012), or in serum-free Gibco StemPro MSC SFM XenoFree Medium (Cat. No. A10675) and Gibco StemPro MSC SFM (Cat. No. A10332). In general, MSCs grow better under hypoxic conditions (2% O2).
How do you characterize human mesenchymal stem cells (MSCs)?

Human MSCs can be identified by flow cytometry, typically displaying CD73+, CD90+, CD105+, CD11b-, and CD45- marker characteristics (Blood 109:4245 (2007)). Human MSC expresses Oct4, Sox2, and Rex-1; these may be verified using RT-PCR (Arthritis Res Ther 9:204 (2007)).
What are mesenchymal stem cells?

Mesenchymal stem cells (MSCs) are multipotent cells isolated primarily from bone marrow or fat tissues that exhibit the ability to differentiate into bone, cartilage, and fat cells. Under normal cell culture conditions, MSCs isolated from bone marrow are spindle shaped with the unique ability to adhere to uncoated plastic culture dishes (Arthritis Res Ther 9:204 (2007)).

For Research Use Only. Not for use in diagnostic procedures.