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Gibco™ CTS™ OpTmizer™ T Cell Expansion SFM, bottle format

Catalog No. A1048501
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A1048501 1000 mL
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Catalog No. A1048501 Supplier Gibco™ Supplier No. A1048501
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Description

CTS™ OpTmizer™ T Cell Expansion SFM, bottle format

GIBCO OpTmizer™ CTS™ T-Cell Expansion SFM has been developed for the growth and expansion of human T lymphocytes. OpTmizer™ CTS™ T-Cell Expansion medium is a complete serum-free, xeno-free 1X medium consisting of OpTmizer™ T-Cell Expansion Basal Medium (1 L bottle) and OpTmizer™ T-Cell Expansion Supplement (26 mL), which are mixed together prior to use.

  • Maintains similar phenotype and function (e.g., cytokine secretion profile) of polyclonally activated and cultured T cells as observed for conventional serum supplemented medium
  • Consistent performance in supporting T cell expansion
  • Supports high density T cell culture (e.g., >3x106 CD3+ T cells/mL) in static culture

    With OpTmizer™ T-Cell Expansion SFM, we offer both basic and clinical research communities a new tool to enhance their success in growing human T-cells. It is specially formulated to allow for superior cell growth & viability, less variability and more consistent results, and easier transition from research to clinical applications.

    Workflow benefits:
  • Minimizing the need to handle purchasing, storage, qualification of serum and thus reducing variability and costs
  • More rapid T cell expansion, thus reducing cycle time and improving productivity
  • Complete serum-free, xeno-free medium, allowing for a seamless transition from research to clinical applications and minimizing regulatory workload

    “This reagent is classified as a medical device in several countries. If it is not regulated as a medical device in specific region, it should be used for laboratory use only.”

    • Specifications

    Cell Type T Cells
    Classification Serum-free, Xeno-free
    Form Liquid
    Product Type Cell Expansion Serum Free Medium (SFM)
    Serum Level Serum-free
    Sterility Sterile
    With Additives HEPES, Phenol Red, Sodium Pyruvate, Sodium Bicarbonate
    Without Additives No Glutamine
    Product Line CTS, OpTmizer
    Quantity 1000 mL
    Endotoxin Level Very Low
    Included Antibiotics No antibiotics
    Inorganic Salts Calcium, Magnesium
    Purity or Quality Grade Research Grade
    Content And Storage OpTmizer™ T-Cell Expansion Basal Medium (1 x 1000 mL bottle): Store at 2–8°C. Protect from light.

    OpTmizer™ T-Cell Expansion Supplement (1 x 26 mL): Store in the dark at 2–8°C.
    Culture Environment CO2
    Culture Type Suspension Cell Culture
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    Frequently Asked Questions (FAQs)

    What is CTS?

    The Gibco Cell Therapy Systems (CTS) portfolio of cell and gene therapy products are GMP manufactured, safety tested, and backed by regulatory documentation to support your transition from discovery through clinical and commercial manufacturing. Through our CTS solutions, we are committed to helping customers streamline therapeutic development, minimize risk, and ease the burden on their quality systems. Learn more here.
    Do I need to order additional components with the CTS OpTmizer T Cell Expansion SFM (Cat. Nos. A1048501, A1048502, A1048503)?

    The CTS OpTmizer T Cell Expansion SFM (Cat. Nos. A1048501, A1048502, A1048503) kit comes with OpTmizer T Cell Expansion Basal Medium and OpTmizer T Cell Expansion Supplement, which are mixed together prior to use. Depending on your protocol you may need to purchase T cell growth factors such as IL-2 (Cat. No. PHC0023) and L-glutamine (Cat. No. 25030) to form a complete medium. To boost growth of human T cells, human AB serum can be added. If desired, antibiotics such as Gentamicin (Cat. No. 15750), can also be added.

    Will CTS OpTmizer T Cell Expansion SFM (Cat. No. A1048501, A1048502, A1048503) work with all types of T cells?

    CTS OpTmizer T Cell Expansion SFM (Cat. No. A1048501, A1048502, A1048503) has been demonstrated to be effective for the ex vivo expansion of human CD3 T cells, including polyclonal T cell populations, antigen-specific T cells, tumor-infiltrating lymphocytes (TIL), cord blood T cells, regulatory T cells, T cells from HIV-infected donors, gene-modified T cells (both lenti- and MLV-based vectors), including cells of both the CD4+ and CD8+ phenotype.

    Can CTS OpTmizer T Cell Expansion SFM (Cat. Nos. A1048501, A1048502, A1048503) be used for animal T cell cultures other than human?

    CTS OpTmizer T Cell Expansion SFM (Cat. No. A1048501, A1048502, A1048503) has only been tested on human T cells.
    Is there a difference in formulation of the CTS OpTmizer T Cell Expansion SFM between the bottle and bag packaging formats?

    The bottle and bag packaging formats of the medium have the same formulation.
    Can I use CTS OpTmizer T Cell Expansion SFM for culture and expansion of mouse T cells? If not, which medium would you recommend?

    We have not tested this medium on mouse T cells. If you're looking for a serum-free medium to culture mouse T cells, you can try AIM V Medium (Cat. No. 12055083 or 12055091). For mouse cells, we recommend adding 50 µM beta-mercaptoethanol (Cat. No. 21985023).

    Can GlutaMAX be used instead of Glutamine to supplement CTS OpTmizer T-Cell Expansion SFM?

    GlutaMAX cannot replace Glutamine because cells may not initially use GlutaMAX so that T cells cannot grow. We recommend adding 4 mM GlutaMAX and 2 mM Glutamine (as stated in the manual) into the CTS OpTmizer T-Cell Expansion SFM if GlutaMAX is needed.
    What is the shelf life of CTS OpTmizer T-Cell Expansion SFM?

    The complete CTS OpTmizer T-Cell Expansion SFM is stable for 4 weeks when stored in the dark at 2-8 degrees C. However, the two individual medium components are stable for 18 months when stored in the dark at 2-8 degrees C.