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Gibco™ CTS™ OpTmizer™ T Cell Expansion SFM, bag format

Catalog No. A1048503
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CTS™ OpTmizer™ T Cell Expansion SFM, bag format

Gibco™ OpTmizer™ CTS™ T-Cell Expansion SFM has been developed for the growth and expansion of human T lymphocytes. OpTmizer™ CTS™ T-Cell Expansion medium is a complete serum-free and xeno-free 1X medium consisting of OpTmizer™ T-Cell Expansion Basal Medium (1 L bag) and OpTmizer™ T-Cell Expansion Supplement (26 mL), which are mixed together prior to use.

  • Maintains similar phenotype and function as T-cells cultured in conventional serum supplemented medium
  • Consistent performance in supporting T-cell expansion
  • Supports high density cultures in static and WAVE Bioreactor™ cultures
  • Be ready for clinical research with this cGmP manufactured formula and support documentation

    Versatile T Cell Culture Media
    OpTmizer™ CTS™ T-Cell Expansion SFM is designed to give results equivalent to conventional serum-supplemented media. However, if desired, 2% heat-inactivated human serum (Cat. No. 34005-100) may be added to the medium to enhance viability and expansion. The use of serum, and the amount required, should be determined empirically depending on the specific T-cell culture application.

    Ready for T Cell Expansion
    OpTmizer™ CTS™ T-Cell Expansion SFM is designed for the expansion of human T-cells permitting CD3+ densities of >3x106 cells/mL in static conditions and >2x107 cells/mL in WAVE bags. Results may vary depending on the T-cell subset (e.g., Ag-specific cells). Conditions for specific T-cell culture applications should be determined empirically.

    Stability of Complete Media
    Complete OpTmizer™ CTS™ T-Cell Expansion SFM (basal, supplement, and L-glutamine) is stable for 4 weeks when stored in the dark at 2–8°C.

    Simplify Your Transition into Clinical Research
    The Gibco™ Cell Therapy Systems (CTS™) brand offers a broad array of products for use in cell therapy applications, including media, reagents, growth factors, enzymes, cell selection/expansion reagents, and devices. Gibco™ CTS™ products deliver:
  • Reduced burden in qualifying reagents
  • Appropriate certificates of analyses and origin included
  • Convenient access to our Drug Master File (DMF)
  • Specifications

    Cell Type T Cells
    Classification Chemically-defined, Serum-free, Xeno-free
    Form Liquid
    Product Type Cell Expansion Serum Free Medium (SFM)
    Serum Level Serum-free
    Sterility Sterile
    With Additives HEPES, Phenol Red, Sodium Pyruvate, Sodium Bicarbonate
    Without Additives No Glutamine
    Quantity 1 Bag
    Endotoxin Level Very Low
    Included Antibiotics No antibiotics
    Inorganic Salts Calcium, Magnesium
    Purity or Quality Grade Research Grade
    Content And Storage OpTmizer™ T-Cell Expansion Basal Medium (1 x L media bag): Store at 2–8°C. Protect from light.

    OpTmizer™ T-Cell Expansion Supplement (1 x 26 mL): Store in the dark at 2–8°C.
    Culture Environment CO2
    Culture Type Suspension Cell Culture
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