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Invitrogen™ CountBright™ Plus Absolute Counting Beads

Catalog No. C36995
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100 tests
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Catalog No. Quantity
C36995 100 tests
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Catalog No. C36995 Supplier Invitrogen™ Supplier No. C36995
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Description

Calibrated suspension of ∼4 μm beads that encapsulate dyes that are excitable across a wide range of UV-IR wavelengths and brightly fluoresce in the emission range of 385-860 nm.

  • Help to determine the count.
  • The concentration of cells in the original sample.
  • Beads are on-scale with most cell types, including human whole lysed blood, for ease of use
  • >95% singlets for improved accuracy
  • Excited by UV-IR lasers for an expanded emission range (385–860 nm)

Specifications

Diameter (Metric) 4 μm
Content And Storage
  • 5 mL beads in buffer
  • Store at 2°C to 8°C
Quantity 100 tests
Shipping Condition Room Temperature
Product Line CountBright
Product Type Cell Counting Bead

Frequently Asked Questions (FAQs)

With the CountBright Plus Absolute Counting Beads/CountBright Absolute Counting Beads, instead of a tight cluster of beads on the scatter plot, I am seeing the beads cover a broad area. What is causing this?

Prior to taking an aliquot, make certain to thoroughly mix the beads. This may also be caused if the beads were ever frozen or the stock solution has any microbial contamination.
Which buffers can I use with the CountBright Plus Absolute Counting Beads and CountBright Absolute Counting Beads?

You may use any physiological buffer or media in a pH range from pH 4 to 10. Avoid extremes of pH and high salt concentrations.
How are CountBright Plus Absolute Counting Beads different from CountBright Absolute Counting Beads?

Here are the differences:

CountBright Plus Absolute Counting Beads:
Bead Size: 4 µm
Excitation Range: 385 - 810 nm
Emission Range: 385 - 860 nm

CountBright Absolute Counting Beads:
Bead Size: 7 µm
Excitation Range: 350 - 635 nm
Emission Range: 350 - 810 nm

For Research Use Only. Not for use in diagnostic procedures.