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Acrobiosystems ACROBiosystems Anti-Cetuximab Antibodies DFS Item

Supplier:  ACROBiosystems Inc CEBY281MG

Antibody;1MG;IgG1/kappa;Recognizes Cetuximab specifically, no cross reactivity with other humanized antibodies.;Mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Cetuximab F(ab')2.; ;Anti-Cetuximab Antibodies bridging ELISA for Anti-Drug Antibody (ADA) assay development. Immobilized cetuximab at 1 g/mL, add increasing concentrations of Anti-Cetuximab Antibodies (Cat. No. CEB-Y28, 10% human serum) and then add biotinylated cetuximab at 5 g/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 2.4 ng/mL. ;Demonstration of the specificity of Anti-Cetuximab Antibodies (Cat. No. CEB-Y28) to the cetuximab.;Anti-Cetuximab Antibodies (mouse IgG1, Cat. No. CEB-Y28) captured on CM5 chip via anti-mouse antibodies surface, can bind Human cetuximab with an affinity constant of 1.51 pM.

Catalog No. 50-195-7068


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