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  6. Invitrogen™ CellROX™ Reagent for oxidative stress detection

Invitrogen™ CellROX™ Reagent for oxidative stress detection

Catalog No. C10443
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C10443 Orange
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Catalog No. C10443 Supplier Invitrogen™ Supplier No. C10443

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

Includes

Five single-use vials with enough total reagent for five 96-well plates or 100 coverslips

CellROX™ Reagents are novel fluorogenic probes for measuring cellular oxidative stress in both live and fixed cell imaging.

The CellROX™ Reagents are novel fluorogenic probes for measuring cellular oxidative stress in live cells. These cell-permeant dyes exhibit bright fluorescence upon oxidation by reactive oxygen species (ROS). (See table below for details of each reagent.)

The CellROX™ reagents are:

  • Optimized for detection of oxidative stress in live cells and may be added directly to serum-containing growth media
  • Provided as a stable, ready-to-use DMSO solutions with simple protocols, compatible with standard workflows in fluorescence microscopy
  • Compatible with a variety of detection platforms, including traditional fluorescence microscopy, high content screening (HCS), and flow cytometry

Oxidative stress results from an imbalance between the production of reactive oxygen species (ROS) and the ability of cells to scavenge them. ROS play an important role in the progression of several diseases, including inflammation, atherosclerosis, aging, and age-related degenerative disorders.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Order Info

Shipping Condition: Wet Ice

Specifications

Color Orange
Format Frozen
Quantity 5 x 50 μL
Content And Storage Store at ≤–20°C. Protect from light and desiccate.
For Use With (Equipment) Imaging, HCS, Cytometer
Concentration 2.5 mM stabilized solution in DMSO
Detection Method Live Cell Imaging
Excitation/Emission 545/565 nm
Indicator Oxidative stress
Product Line CellROX

Frequently Asked Questions (FAQs)

What dyes can I use to detect reactive oxygen species (ROS) in my bacteria?

Many dyes that are used on mammalian cells have also been shown to be useful in bacterial cells. For example, CellROX Deep Red Reagent has been shown to work in B. subtilis (see Reference: http://www-brs.ub.ruhr-uni-bochum.de/netahtml/HSS/Diss/RaatschenNadja/diss.pdf). If you are interested in a particular dye, but are not sure if it will work on your bacteria, literature searches are the best way to check to see if it has been tested. If not, then it may be worth testing yourself.

I am trying to label my cells with a reactive oxygen species (ROS) indicator dye, but I am not seeing a significant difference in signal. What could be happening?

First, make sure you have both a negative (untreated) and positive (ROS-induced) sample to compare. A good positive control can be the use of 100 µM menadione for one hour or 50 µM nefazodone for 24 hours. H2O2 can also be used, though it does not work well for CellROX dyes. Some dyes, such as H2DCFDA, require esterase cleavage, so don't incubate in the presence of serum (which contains esterases that can prematurely cleave the dye). If your positive control does not show significant change compared to the negative control, try increasing the concentration and label time for the dye. Our manuals give starting recommendations. Be sure to image your live cells as soon as possible. Only two dyes (CellROX Green and CellROX Deep Red) are retained with formaldehyde fixation. Finally, make sure you are using filters and instrument settings to match the excitation and emission spectra of the dye.

For Research Use Only. Not for use in diagnostic procedures.