Sigma-Aldrich Bouin’s Solution

Bouin's solution is a noncoagulant yellow stain fixative. It imposes shrinkage on tissues when used as preservative due to its picric acid component. It is majorly used in ureteroscopic biopsies. It preserves morphological and nuclear information in tissues upon fixation. Bouin's solution has been used: in the staining of human liver biopsy tissue samples for two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) imaging, in the fixation of rat ovaries for the observing follicle cells using microscopy, in the fixation of gonads of crab for morphological and histological analysis. Prepared with saturated picric acid, formaldehyde and acetic acid. Excellent fixative for preserving soft and delicate structures.

• Used as a mordant in various trichrome procedures
• Shelf life: expiry date on the label
• Composition: acetic acid (5%), formaldehyde (9%), and picric acid (0.9%)
• Color: yellow
• Applications: hematology and histology.
• Storage temperature: room temperature