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MilliporeSigma™ Blood Vessel Staining Kit

Catalog No. ECM590
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ECM590 1 Kit
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Catalog No. ECM590 Supplier MilliporeSigma™ Supplier No. ECM590

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

Includes

  • Rabbit anti-vWF Polyclonal Antibody: One vial containing 100μg at 1mg/mL
  • Mouse anti-CD31 Monoclonal Antibody: One vial containing 100μL at 15mg/mL (total protein including IgG and Carrier protein)
  • Goat anti-Rabbit Secondary Antibody: One 15mL ready-to-use solution containing biotinylated goat anti-rabbit IgG in PBS with a carrier protein
  • Goat anti-Mouse Secondary Antibody: One 15mL ready-to-use solution containing biotinylated goat anti-mouse IgG in PBS with a carrier protein
  • Blocking Reagent: One 15mL ready-to-use solution containing normal goat serum in PBS with a carrier protein
  • Streptavidin-HRP: One 15ml ready-to-use solution of Streptavidin-HRP diluted in Tris-buffered saline (TBS)
  • DAB Chromogen A: One 5mL bottle of 3, 3' Diaminobenzidine diluted in TBS
  • DAB Chromogen B: Two 12mL bottles of hydrogen peroxide diluted in distilled water
  • 20X Rinse Buffer: One 275mL bottle of 20X TBS

Very sensitive immunohistochemical tool for the detection of vascular endothelial cells and provides simple, all-in-one method for staining of frozen and paraffin-embedded sections in human (both CD31 and VWF) or rat and mouse (VWF only) tissues

  • Reagents and materials supplied in the Blood Vessel Staining Kit are sufficient for staining 100 tissue sections and when stored at 2-8ºC is stable up to the expiration date
  • ECM590 detects vWF and CD31 in human paraffin embedded tissues
  • In rat or mouse tissues only the vWF antibody is effective in paraffin; the CD31 antibody does not react with rodent CD31

Specifications

Content And Storage Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date. Do not store kit components or perform staining in strong light, such as direct sunlight
Detection Method Chromogenic
For Use With (Application) Immunohistochemistry, Immunohistochemistry (Paraffin)
Quantity 1 Kit