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MilliporeSigma™ Blood Vessel Staining Kit
Very sensitive immunohistochemical tool for the detection of vascular endothelial cells and provides simple, all-in-one method for staining of frozen and paraffin-embedded sections in human (both CD31 and VWF) or rat and mouse (VWF only) tissues
Supplier: MilliporeSigma™ ECM590
Includes:
- Rabbit anti-vWF Polyclonal Antibody: One vial containing 100μg at 1mg/mL
- Mouse anti-CD31 Monoclonal Antibody: One vial containing 100μL at 15mg/mL (total protein including IgG and Carrier protein)
- Goat anti-Rabbit Secondary Antibody: One 15mL ready-to-use solution containing biotinylated goat anti-rabbit IgG in PBS with a carrier protein
- Goat anti-Mouse Secondary Antibody: One 15mL ready-to-use solution containing biotinylated goat anti-mouse IgG in PBS with a carrier protein
- Blocking Reagent: One 15mL ready-to-use solution containing normal goat serum in PBS with a carrier protein
- Streptavidin-HRP: One 15ml ready-to-use solution of Streptavidin-HRP diluted in Tris-buffered saline (TBS)
- DAB Chromogen A: One 5mL bottle of 3, 3' Diaminobenzidine diluted in TBS
- DAB Chromogen B: Two 12mL bottles of hydrogen peroxide diluted in distilled water
- 20X Rinse Buffer: One 275mL bottle of 20X TBS
Description
- Reagents and materials supplied in the Blood Vessel Staining Kit are sufficient for staining 100 tissue sections and when stored at 2-8ºC is stable up to the expiration date
- ECM590 detects vWF and CD31 in human paraffin embedded tissues
- In rat or mouse tissues only the vWF antibody is effective in paraffin; the CD31 antibody does not react with rodent CD31
Specifications
| Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date. Do not store kit components or perform staining in strong light, such as direct sunlight | |
| Chromogenic | |
| Immunohistochemistry, Immunohistochemistry (Paraffin) | |
| 1 Kit |