MP Biomedicals Betaine 5M

Description

MP Biomedicals Betaine (5 M, 2 mL) is a ready-to-use PCR-grade reagent formulated to enhance nucleic acid amplification performance, particularly for GC-rich or structurally challenging templates. As a methylated glycine analogue, betaine modulates DNA duplex stability by minimizing secondary structures, facilitating primer annealing, and improving polymerase processivity under adverse conditions. This additive is particularly useful when standard PCR conditions yield poor specificity or low yields due to problematic regions such as high GC content or stable hairpins. It functions by equalizing the thermal denaturation behavior of AT- and GC-rich sequences and by stabilizing the DNA polymerase in variable local environments of duplex melting. Importantly, the reagent is supplied as a sterile or non-sterile (as indicated in product specs) 5 M aqueous solution that may be added directly to PCR mixes without further dilution, saving preparation time.

Its technical features and benefits include:

• Enhanced amplification yield and specificity in difficult templates (especially GC-rich or structured targets)
• Reduced formation of primer-dimers and non-specific amplification through stabilization of annealing dynamics
• Compatibility with standard, high-fidelity, and quantitative PCR (qPCR) methods when combined with polymerases and buffers
• Facilitates downstream applications including cDNA synthesis, sequencing of GC-rich regions, and long-amplicon PCR
• Supplied in a convenient 2 mL format to allow precise volumetric addition and minimize reagent waste
• Molecular biology grade with rigorous QC to minimize impurities that might inhibit polymerases or downstream processes
• Recommended storage at +4°C to preserve reagent integrity over time.
• Use this Betaine reagent as a supplemental additive in your PCR or amplification protocols to rescue problematic amplifications and improve consistency across challenging templates