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Gibco™ BenchStable™ RPMI 1640
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Catalog No. A4192302
Encompass
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Description

Gibco RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) was originally developed to culture human leukemic cells in suspension and as a monolayer.

Gibco RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

BenchStable RPMI 1640 Medium has been engineered for storage at room temperature while supporting cellular proliferation and maximum cell densities comparable to conventional RPMI 1640 Medium when supplemented with ≥10% fetal bovine serum (FBS).

This RPMI 1640 Medium is modified as follows:
• Optimized for room temperature stability
• With GlutaMAX, Phenol Red
• Without HEPES

Using BenchStable RPMI 1640 Medium
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L) and so requires a 5–10% CO2 environment to maintain physiological pH. RPMI 1640 Medium with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. BenchStable RPMI 1640 Medium contains no proteins, lipids, or growth factors and so requires supplementation with ≥10% fetal bovine serum (FBS).

Specifications

Cell Line HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Classification Animal Origin-free
Concentration 1 X
Form Liquid
Product Type RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Serum Level Standard Serum Supplementation
Sterility Sterile-filtered
With Additives GlutaMAX, Phenol Red, Phenol Red
Without Additives No HEPES, No Sodium Pyruvate
Manufacturing Quality cGMP-compliant under the ISO 9001: 2015 standard
Product Line BenchStable
Quantity 10 x 500 mL
Shipping Condition Room Temperature
Green Features ACT Ecolabel (Silver)
Content And Storage Store at room temperature. Protect from light.
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Frequently Asked Questions (FAQs)

Is it okay if I supplement BenchStable media with more than 10% FBS?

We recommended supplementing BenchStable media with at least 10% FBS for best results. If your culture method calls for greater than 10% FBS supplementation using our other existing media, we recommend that you use the same concentration with BenchStable media.
Is it okay if I supplement BenchStable media with 5% FBS?

We recommend supplementing BenchStable media with at least 10% FBS for best results. We have run tests on limited cell types using 5% FBS concentration and have not observed compromised performance. Please contact Tech Support at techsupport@thermofisher.com to ensure that your specific application allows for 5% serum supplementation.
Which cell lines have been tested in BenchStable media?

We have tested our BenchStable media for growth and morphology on the following cell lines: A549, CHO-K1, HEK293, HeLa, HL-60, Jurkat, MCF7, MDA-MB-231, Neuro2A, SH-SY5Y, and THP-1.
Do you offer BenchStable media without phenol red?

At this time, we do not offer BenchStable media without phenol red.
Will the shelf-life of BenchStable media improve if stored at lower temperature (2-8 degrees C)?

The shelf life of BenchStable media is unchanged when stored cold (2-4 degrees C) or ambient (15-30 degrees C).

How should I store BenchStable media? Can they be stored at 4 degrees C?

We recommend storing BenchStable media under ambient conditions (15-30 degrees C), protected from light. BenchStable media can alternatively be stored at 2-8 degrees C.
Can I store the box containing BenchStable medium in the refrigerator?

Yes, the box provided with our BenchStable media has properties that offer some resistance to moisture generated in a refrigerator environment.
My BenchStable medium arrived partially/completely frozen. Is it still stable?

Yes, BenchStable media should be stable for use if received partially or completely frozen.
Is degradation of BenchStable media due to light exposure only limited to fluorescent light?

Our in-house data, using refrigerated BenchStable media exposed to fluorescent light (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/classical-media/benchstable-cell-culture-media.html#results), illustrates the necessity of having light protection. While we have not tested other light spectrums; we recommend storing BenchStable media protected from any light exposure.

How long can BenchStable media be exposed to light before cell growth is affected?

We have observed rapid degradation of several components within days from exposure of BenchStable media to common fluorescent light. We have also observed decreased cell growth using light-exposed media.

What is the recommended usage timeframe for BenchStable media after serum supplementation?

BenchStable media can be used for up to 2-4 weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.
How long are BenchStable media good for after the bottle has been opened?

To reduce the chances of contamination and the impact of pH drift, we recommend using BenchStable media within 2-4 weeks after the bottle has been opened.
How should I store complete or serum-supplemented BenchStable media?

Complete BenchStable media (for example, media supplemented with 10% FBS) should be stored at 4 degrees C.
What is the shelf-life of BenchStable media?

BenchStable media have a 12 month shelf life, consistent with our standard basal media offerings.

For Research Use Only. Not for use in diagnostic procedures.