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  5. Invitrogen™ Armenian Hamster IgG Isotype Control (eBio299Arm), Super Bright™ 436, eBioscience™

Invitrogen™ Armenian Hamster IgG Isotype Control (eBio299Arm), Super Bright™ 436, eBioscience™

Catalog No. 62488882
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100 μg
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62488882 100 μg
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Catalog No. 62488882 Supplier Invitrogen™ Supplier No. 62488882
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Description

Armenian Hamster Monoclonal Isotype Control

The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin. Applications Reported: This eBio299Arm antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio299Arm antibody has been tested by flow cytometric analysis of mouse splenocytes. Use isotype control at the same concentration as experimental antibody. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Specifications

Antigen Armenian Hamster IgG
Applications Control, Flow Cytometry
Classification Monoclonal
Clone eBio299Arm
Concentration 0.2 mg/mL
Conjugate Super Bright 436
Formulation PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias IgG; Immunoglobulin G; ImmunoglobulinG
Host Species Armenian Hamster
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Isotype Control (Primary)
Target Species Not Applicable
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Isotype Control
Form Liquid
Isotype IgG
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