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Applied Biosystems™ ViiA™ 7 Array Card RNaseP Verification Kit

Catalog No. 4432265
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Catalog No. 4432265 Supplier Applied Biosystems™ Supplier No. 4432265
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Description

Includes

An empty TaqMan Low Density Array and eight vials of RNase P detection reagents to detect and quantitate genomic copies of the human RNase P gene. Each vial contains premixed reagents (1X TaqMan Universal PCR Master Mix, RNase P primers, and FAM™ dye-labeled probe) and template.

Used to validate the performance of the ViiA™ 7 Real-Time PCR System with the TaqMan™ Low Density Array Block Upgrade.

The ViiA™ 7 System TaqMan™ Low Density Array Instrument Verification RNase P kit contains an empty TaqMan™ Low Density Array and 8 vials of RNase P detection reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each vial contains premixed reagents (1X TaqMan™ Universal PCR Master Mix, RNase P primers, and FAM™ dye-labeled probe) and template. The ViiA™ 7 System TaqMan™ Low Density Array Instrument Verification RNase P kit must demonstrate the ability to distinguish between 5,000 and 10,000 genomic equivalents with a 99.7% confidence level for a subsequent sample run in a single well. To ensure accurate results, Applied Biosystems™ recommends that the performance of your instrument be verified every six months.

The kit must demonstrate the ability to distinguish between 5000 and 10,000 genomic equivalents with a 99.7% confidence level for a subsequent sample run in a single well. To ensure accurate results, the instrument should be verified every six months.

Order Info

Store kit at -15° to -25°C.;

Specifications

Format 384-well Microfluidic Card
Content And Storage 8 x Tubes with Standard Dilutions, Empty Array Card, Port 1 NTC, Port 2 Unknown A, Port 3 U, Store at -15°C to -25°C
For Use With (Equipment) ViiA™ 7 System
Product Line ViiA
Product Type Array Card RNaseP Verification Kit
For Use With (Application) Real Time PCR (qPCR)
Quantity 1 kit
Calibration Type RNase P Calibration, Instrument Verification Plate

Frequently Asked Questions (FAQs)

When do I need to run the RNaseP calibration?

The RNaseP verification plate contains template, master mix, and a TaqMan assay for RNaseP. It is used to verify that the instrument is performing to specifications. If you have reason to suspect there is something wrong with the instrument, or if you want to rule out a chemistry issue, the RNaseP plate is a good way to test the system. The RNaseP verification plate is a single-use plate.

When do I need to run the RNaseP calibration for the QuantStudio 12K Flex Real-Time PCR System?

The RNaseP verification plate contains template, master mix, and a TaqMan assay for RNaseP. It is used to verify that the instrument is performing to specifications. If you have reason to suspect there is something wrong with the instrument, or if you want to rule out a chemistry issue, the RNaseP plate is a good way to test the system. The RNaseP verification plate is a single-use plate.

I cannot open the QuantStudio 3/5 Design & Analysis 2 (DA2) software on my Mac. What are the software requirements for Mac?

To open the software download on your Mac, you will need to adjust your security settings using the following instructions from Apple support:

Safely open apps on your mac

Please note, the software should run on Mac OS 10.10 or later.

Can I import a standard curve for my real-time PCR data into the Design & Analysis Software (v2.x)?

It is possible to import a standard curve into the Design & Analysis Software (v2.x) if you are analyzing results from one of the following Applied Biosystems real-time PCR instruments:
• 7900HT Fast Real-Time PCR System
• 7500/7500 Fast Real-Time PCR System
• StepOne/StepOne Plus Real-Time PCR System
• ViiA 7 Real Time PCR System
• QuantStudio 1 Real-Time PCR System
• QuantStudio 3 & 5 Real-Time PCR System
• QuantStudio 6 & 7 Flex Real-Time PCR System
• QuantStudio 6 & 7 Pro Real-Time PCR Systems
• QuantStudio 12K Flex Real-Time PCR system

Follow the instructions below to import a standard curve for analysis in the Design & Analysis Software (v2.x):
1. Open the data file in the Design & Analysis Software (v2.x).
2. Click on Actions.
3. Click on "Standard Curve Analysis Setting".
4. Select "External Standard Curves" and click on "Import".
5. Browse to the proper .csv file and click "Open".
6. Click "Apply".

Can I import a standard curve for my real-time PCR data into the QuantStudio Real-Time PCR Software?

If you are analyzing results from the ViiA 7 Real-Time PCR System, QuantStudio 6 Flex Real-Time PCR System, or QuantStudio 7 Flex Real-Time PCR System, it is possible to import a standard curve into the QuantStudio Real-Time PCR Software following the instructions below:
1. Open the data file in the QuantStudio Real-Time PCR Software.
2. Go to Analysis Settings.
3. Click on the "Standard Curve Settings" tab.
4. Select the option to "Use a standard curve imported from another experiment" and click on "Import".
5. Browse to and highlight the proper data file and click "Select".
6. Click Apply Analysis Settings.
How can I get a summary of the calibrations performed on the QuantStudio 6 and 7 Flex Real-Time PCR Systems?

You can get a summary of the calibrations from the QuantStudio Software by going to Instrument > Instrument Console > Manage Instrument > Information > Print Calibration Log.

Do I need to back up my calibration files before I upgrade the software for the ViiA7 Real-Time PCR System?

No, you do not need to back up the calibration files as all of the calibration files are stored on the ViiA7 Real-Time PCR instrument. Once the computer software is properly installed and the computer is connected to the instrument, the calibration information will automatically update in the QuantStudio Software.
Can I still start a run on the ViiA7 Real-Time PCR System if the "Calibration is expired" notification is displayed?

Yes, you can still start a run on the ViiA7 Real-Time PCR System if the expired calibration notice displays. This is just a reminder and you can close it to proceed with the run. Additionally, you can change when you want the notifications to show up and how frequently you want to be notified to calibrate the instrument. To do this, go to the toolbar in the QuantStudio Software and select Instrument > Instrument Console. Select your instrument icon and click on Manager Instrument > Maintenance > Calibration Reminders. From the Calibration reminders Settings page you can update when the calibrations expire, turn expiration reminders on/off, and set when the reminders show up.

For Research Use Only. Not for use in diagnostic procedures.