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Applied Biosystems™ StepOnePlus™ Real-Time PCR System Spectral Calibration Kit (96 well)

Catalog No. 4371435
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Catalog No. 4371435 Supplier Applied Biosystems™ Supplier No. 4371435
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Description

Includes

Includes one background plate and two 96-well fast reaction plates containing spectra dyes (plates I & II). Plate I contains FAM, VIC, NED, and ROX; Plate II contains SYBR Green I, JOE, and TAMRA.

Easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR™ Green I, VIC™/JOE™, and NED™/TAMRA™/ROX™ dyes on the StepOnePlus™ RT-PCR System.

The Applied Biosystems™ StepOnePlus™ Real-Time PCR System Spectral Calibration Kit contains one background plate and two preloaded, 96-well fast optical reaction plates containing seven dye standards.

This easy-to-use kit enables users to calibrate the StepOnePlus Real-Time PCR System quickly and easily. Insert the reaction plates during system installation, and the system automatically collects the dye standards' spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

  • Provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture
  • Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions

Order Info

Dry ice shipping; store at -15° to -25°C; protect from light

Specifications

Content And Storage Includes one background plate and two 96-well fast reaction plates containing spectra dyes (plates I and II). Plate I contains FAM, VIC, NED, and ROX; Plate II contains SYBR Green I, JOE, and TAMRA.

Store at -15°C to -25°C. Protect from light.
Calibrated Dye FAM, JOE, NED, ROX, TAMRA™, VIC, SYBR™ Green
For Use With (Equipment) Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode
Detection Method Fluorescence
Product Line StepOnePlus
Product Type Spectral Calibration Kit
For Use With (Application) Real Time PCR (qPCR)
Shipping Condition Dry Ice
Quantity 1 Kit
Calibration Type Spectral Calibration

Frequently Asked Questions (FAQs)

What type of Real-Time PCR (Sequence Detection System) information is available from Applied Biosystems?

Tutorials demonstrating introductions to Real-Time PCR, guidelines for designing real-time assays using Primer Express software, and various instrument set-ups can be found under Life Technologies University on our website. User bulletins, manuals and Product inserts, protocols can be found as downloadable pdf files on our Technical Resources page.

For Research Use Only. Not for use in diagnostic procedures.