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Applied Biosystems™ StepOnePlus™ Real-Time PCR System Spectral Calibration Kit (96 well) DFS Item

Easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR™ Green I, VIC™/JOE™, and NED™/TAMRA™/ROX™ dyes on the StepOnePlus™ RT-PCR System.

Manufacturer:  Applied Biosystems™ LS4371435

Catalog No. 4371435



Includes: Includes one background plate and two 96-well fast reaction plates containing spectra dyes (plates I & II). Plate I contains FAM, VIC, NED, and ROX; Plate II contains SYBR Green I, JOE, and TAMRA.

Description

Description

The Applied Biosystems™ StepOnePlus™ Real-Time PCR System Spectral Calibration Kit contains one background plate and two preloaded, 96-well fast optical reaction plates containing seven dye standards.

This easy-to-use kit enables users to calibrate the StepOnePlus Real-Time PCR System quickly and easily. Insert the reaction plates during system installation, and the system automatically collects the dye standards' spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

  • Provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture
  • Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions

Order Info

Dry ice shipping; store at -15° to -25°C; protect from light

Specifications

Specifications

Includes one background plate and two 96-well fast reaction plates containing spectra dyes (plates I & II). Plate I contains FAM, VIC, NED, and ROX; Plate II contains SYBR Green I, JOE, and TAMRA.

Store at -15°C to -25°C. Protect from light.
FAM, JOE, NED, ROX, TAMRA™, VIC, SYBR® Green
Dry Ice
Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode
Fluorescent
StepOnePlus™
Spectral Calibration
SDS
Documents

For Research Use Only. Not for use in diagnostic procedures.