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Thermo Scientific™ Aminoallyl-dUTP Solution (10 mM)

Catalog No. FERR0091
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100 μL
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FERR0091 100 μL
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Catalog No. FERR0091 Supplier Thermo Scientific™ Supplier No. R0091
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Description

Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH.

Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH.

Aminoallyl-dUTP can be enzymatically incorporated into DNA with:
• Reverse transcriptases
• Taq DNA polymerase (Cat. No. EP0072)
• phi29 DNA Polymerase (EP0091)
• Klenow Fragment (EP0051)
• Klenow Fragment, exo- (EP0421)
• DNA Polymerase I (EP0041)

The resulting amine-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin, or hapten.

Applications
• Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis
• PCR
• Nick-translation
• Random-primed labeling
• Primer extension

Specifications

Concentration 10 mM
Content And Storage Aminoallyl-dUTP Solution, 10 mM (100 μL)

Store at –20°C.
Form Liquid
Polymerase DNA Polymerase
Labeling Method Direct Labeling
Label or Dye Aminoallyl
Quantity 100 μL
Shipping Condition Dry Ice
For Use With (Application) cDNA Synthesis, Non-Radioactive Labeling

For Research Use Only. Not for use in diagnostic procedures.