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Thermo Scientific™ Aminoallyl-dUTP


Perform enzymatic indirect non-radioactive labeling of DNA with the modified nucleotide Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate).

Manufacturer: thermo scientific™  R0091

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Catalog No. FERR0091

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Description & Specifications

Specifications

For Use With (Application) Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random primed labeling or primer extension.
Quantity 1µmol (0.1mL of 10mM solution)

Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH.

Aminoallyl-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, Klenow Fragment, exo- and DNA Polymerase I. The resulting amine-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten.

Aminoallyl-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, Klenow Fragment, exo- and DNA Polymerase I. The resulting amine-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten.

Recommended For:

Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random primed labeling or primer extension.