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Invitrogen™ Poly(A) Polymerase (cloned) 2 U/μL

Catalog No. AM2030
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Quantity:
80 units, 2 μL
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Catalog No. Quantity
AM2030 80 units, 2 μL
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Catalog No. AM2030 Supplier Invitrogen™ Supplier No. AM2030
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Description

Includes

Poly(A) Polymerase, 5X Poly(A) Polymerase Buffer, and 25mM MnCl2

Catalyzes the addition of adenosine to the 3' end of RNA in a sequence-independent fashion

  • The Invitrogen Poly(A) Polymerase enzyme can be used to append radiolabeled ATP to the 3' end of RNA molecules to generate labeled RNAs
  • One tube containing 80U (2U/μL), 5X Poly(A) Polymerase Buffer, and 25mM MnCl2 are provided
  • The enzyme can be used in conjunction with terminal transferase, reverse transcriptase, and thermostable polymerase to clone RNAs of unknown sequence
  • Unit Definition: One unit is the amount of enzyme required to incorporate 1nmol of AMP into acid-insoluble material in 10 minutes at 37°C

DNA & RNA Purification & Analysis, Nucleic Acid Labeling & Oligo Synthesis, PCR & Real-Time PCR, RNA Labeling, Reverse Transcription

Order Info

Shipping Condition: Dry ice

Specifications

Content And Storage Poly(A) Polymerase, 5X Poly(A) Polymerase Buffer, and 25 mM MnCl2 should be stored at –20°C.
Shipping Condition Dry Ice
Polymerase Poly(A) RNA Polymerase
Product Line Ambion
Quantity 80 units, 2 μL
Concentration 5X
Source RNA

Frequently Asked Questions (FAQs)

Is the Poly(A) Polymerase (cloned) (Cat. No. AM2030) from E. coli?

PolyA polymerase (cloned) is purified from an E. coli strain overexpressing E. coli Poly(A) Polymerase I. Here is the link to the product information sheet: https://assets.thermofisher.com/TFS-Assets/LSG/manuals/4393885_PlyAPlymrseCln_PI.pdf

For Research Use Only. Not for use in diagnostic procedures.