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Invitrogen™ MEGAclear™ Transcription Clean-Up Kit

Quickly cleanup large-scale transcription reactions

Supplier: Invitrogen™ AM1908

Catalog No. AM1908

$235.00 / Each

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Quantity

Description

MEGAclear Kit is designed for quick cleanup of any large-scale transcription reaction. MEGAclear Kit efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components.

Purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components
High recovery from 1ng to 500μg of input
Ideal for purification of aRNA amplification reactions, nonisotopically labeled RNA probes, and capped RNA transcripts for oocyte injections
Quick protocol—purify RNA in less than 30 minutes
Using the MEGAclear Kit: Protocol is quick and easy. Transcription reaction is mixed with Binding Solution, passed over an RNA-binding filter, and washed; an elution step releases the purified RNA, which is ready for any application requiring highly pure synthetic or in vitro-transcribed RNA. For RNA recovery of 70% or greater, single-stranded RNA should be >100 nt and double-stranded RNA should be >200 bp.

Specifications

Content And Storage	Binding Solution, Wash Solution Concentrate, and 5 M Ammonium Acetate are stored at 4°C. Elution Solution can be stored at any temperature, and Filter Cartridges and Collection and Elution Tubes are stored at room temperature.
Format	Tube
Purification Target	mRNA, Synthetic mRNA
For Use With (Application)	In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
High-throughput Compatibility	Not High-throughput Compatible (Manual)
Product Line	Ambion™, MEGAclear™
Quantity	20 Preps
Shipping Condition	Room Temperature
Yield	500 μg

Final Product Type	Purified Synthetic mRNA, Purified mRNA
Isolation Technology	Silica Spin Column
Sample Type	mRNA, Synthetic mRNA
For Use With (Equipment)	Vacuum Manifold, Centrifuge
No. of Reactions	20 Preps
Product Type	mRNA purification kit, Purification kit
Purification Time	30 min
Starting Material Volume	1 ng to 500 μg of dsRNA>200b or ssRA>100bp

Product Suggestions Documents

Documents

Product Information

Manuals

Protocol: MEGAclear™ Kit: Purification for Large Scale Transcription Reactions (English ) Version: 1908M Rev. C 02.2013 File size: 318.9 kB

For Research Use Only. Not for use in diagnostic procedures.