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Invitrogen™ MAXIscript™ T7/T3 Transcription Kit DFS Item

Fast synthesis of RNA probes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations

281,37 $ - 532,00 $

Spécifications

Content And Storage T7 Enzyme Mix, T3 Enzyme Mix,10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Final Product Type Synthetic mRNA, mRNA
Format Kit
Sample Type mRNA, Synthetic mRNA
For Use With (Application) In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
voir les spécifications

Comprend: T7 Enzyme Mix, T3 Enzyme Mix, 10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, pTRI-b-actin, Nuclease-free Water

Produits 2
Numéro de catalogue Numéro du manufacturier. Product Type No. of Reactions Prix Quantité  
Numéro de catalogue Numéro du manufacturier. Product Type No. of Reactions Prix Quantité  
AM1326 DFS Item
Afficher les documents
Invitrogen™
AM1326
T7/T3 In Vitro Transcription kit 100 Reactions N/A
Il en reste null
 
AM1324 DFS Item
Afficher les documents
Invitrogen™
AM1324
T7/T3 In Vitro Transcription kit 30 Reactions N/A
Il en reste null
 
Description

Description

The Ambion MAXIscript T3/T7 In Vitro Transcription Kit synthesizes RNA probes in just 10 minutes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations. Each kit contains sufficient reagents for 15 reactions of each enzyme. MAXIscript Kits are very efficient at synthesizing full-length probes, even at limiting nucleotide concentrations, making them the perfect companion to ribonuclease protection assays. MAXIscript Kits are also an excellent choice for the synthesis of nonisotopically labeled probes. Labeling can be accomplished post-transcriptionally by using Ambion's BrightStar™ Psoralen-Biotin Labeling Kit, or during transcription by incorporating modified nucleotides into the reaction. MAXIscript Kits can also be used for the synthesis of modest amounts of unlabeled RNA (2–6μg/20μL reaction) of up to 2kb.

  • High yields of high specific activity, full-length RNA probes in just 10 minutes
  • Ideal probe synthesis kit for nuclease protection assays, Northern and Southern blots, and in situ hybridization
  • Includes TURBO DNase™
  • All kits contain quality tested DNase/RNase-free reagents
  • The MAXIscript In Vitro Transcription Kit synthesizes RNA probes with specific activities reaching 1 ×109 cpm/μg in just 10 minutes

Spécifications

Spécifications

T7 Enzyme Mix, T3 Enzyme Mix,10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Kit
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
T7/T3 In Vitro Transcription kit
Dry Ice
PCR Products, Linearized DNA plasmid
Synthetic mRNA, mRNA
mRNA, Synthetic mRNA
Ambion™, MAXIscript™
T3, T7
2-6 μg
Videos
FDS
missing translation for 'documents'

missing translation for 'documents'

For Research Use Only. Not for use in diagnostic procedures.