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Invitrogen™ Ambion™ MAXIscript™ T3 Transcription Kit DFS Item


Fast synthesis of RNA probes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations

Manufacturer: invitrogen™  AM1316

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Catalog No. AM1316

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Description & Specifications

Specifications

For Use With (Application) DNA and RNA Purification and Analysis, Gene Expression Analysis and Genotyping, in vitro Transcription, Northern Blotting, Nuclease Protection Assays, Nucleic Acid Gel Electrophoresis and Blotting, Southern Blotting
Includes T3 Enzyme Mix, 10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, pTRI-b-actin, and Nuclease-free Water
Product Type Kit
Quantity 30 reactions
Storage Requirements T3 Enzyme Mix, 10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at -20°C. Nuclease-free Water may be stored at any temperature.

The Ambion MAXIscript T3 In Vitro Transcription Kit synthesizes RNA probes in just 10 minutes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations. Each kit contains sufficient reagents for 30 reactions. The MAXIscript In Vitro Transcription Kit synthesizes RNA probes with specific activities reaching 1 × 109 cpm/μg in just 10 minutes. MAXIscript Kits are very efficient at synthesizing full-length probes, even at limiting nucleotide concentrations, making them the perfect companion to ribonuclease protection assays. MAXIscript Kits are also an excellent choice for the synthesis of nonisotopically labeled probes. Labeling can be accomplished post-transcriptionally by using Ambion's BrightStar™ Psoralen-Biotin Labeling Kit, or during transcription by incorporating modified nucleotides into the reaction. MAXIscript Kits can also be used for the synthesis of modest amounts of unlabeled RNA (2 to 6μg/20μL reaction) of up to 2kb.

  • High yields of high specific activity, full-length RNA probes in just 10 minutes
  • Ideal probe synthesis kit for nuclease protection assays, Northern and Southern blots, and in situ hybridization
  • Includes TURBO DNase™
  • All kits contain quality-tested DNase/RNase-free reagents
  • RNA Yield: 2 to 6μg