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Invitrogen™ Ambion™ In Vivo GAPDH Positive Control siRNA
Description
What are Invitrogen™ In Vivo siRNAs?
Invitrogen™ In Vivo siRNAs are designed using the proven Silencer™ Select algorithm and incorporate chemical modifications for superior serum stability with in vivo delivery. The added serum stability does not compromise the performance of the siRNAs. Invitrogen™ In Vivo siRNAs have been shown to be non-toxic in vivo (mouse) and non-immunogenic (cell-based assays). In cell-based assays, Invitrogen™ In Vivo siRNAs exhibit equivalent or better potency than Silencer™ Select siRNAs. The result is better confidence in your in vivo RNAi experiments, better knockdown when paired with an efficient delivery solution, and the assurance of limited non-specific effects.
GAPDH Positive Control siRNAs
Our extensively validated, positive control siRNA to human, mouse, and rat GAPDH serves multiple functions. First, it is an ideal “test” siRNA for those just beginning siRNA experiments, because it is validated to work in multiple cell lines. In addition, because it targets GAPDH mRNA, which is commonly used as an internal control, its effects are easy to assay, and thus provides an excellent tool to monitor siRNA transfection efficiency by real-time RT-PCR. The Invitrogen™ In Vivo GAPDH Positive Control siRNA shows increased serum and nuclease resistance compared with Silencer™ Select siRNAs without loss of potency or specificity and is recommended for in vivo applications.
Quality Control
Invitrogen™ synthesizes and purifies each Invitrogen™ In Vivo siRNA in state-of-the-art facilities to meet the highest quality standards. As part of our rigorous quality control procedures, each RNA oligonucleotide is analyzed by MALDI-TOF mass spectrometry, and analytical HPLC is used to monitor purity. To provide the utmost in quality, we also assess each annealed siRNA by gel electrophoresis to confirm that the strands anneal properly. The result is premium-quality siRNA that is purified and ready to use.
In Vivo Ready Controls
Our In Vivo Ready Control siRNAs are subjected to that extra level of purification and testing required for the introduction of siRNAs into animals. After HPLC purification and annealing, each siRNA is further purified utilizing a process that removes excess salt via a semi-permeable membrane. The result is highly pure siRNA with minimal salt content, suitable for in vivo applications. In vivo siRNAs are then sterile filtered, and tested for the presence of endotoxin. At concentrations of 50μM in presence of deionized water, in vivo Ready siRNAs contain <5.0 mM Na+, <0.06 mM K+, and <0.02 mM Mg2+.
Specifications
Specifications
| Content And Storage | The siRNA (annealed) is shipped at ambient temperature as dried down oligonucleotides in a single tube, along with 1.75 ml Nuclease-free Water for resuspension. Store at or below –20°C. |
| Control Type | Positive Control |
| RNAi Type | siRNA |
| For Use With (Application) | RNAi, Transfection |
| Label or Dye | Unconjugated |
| Product Line | Ambion |
| Product Type | siRNA |
| Purity | In Vivo, HPLC |
| Quantity | 5 nmol |
| Shipping Condition | Room Temperature |
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Frequently Asked Questions (FAQs)
- 100x more stable than unmodified siRNA - longer knockdown in vivo
- Compatible with any 21-mer sequence - no loss of potency
- Non-toxic - actually reduces siRNA sequence specific immune responses
- Enhanced specificity - up to 90% reduction in off target effects
- Both Pre-designed and Validated Invitrogen In Vivo siRNAs offered
Please see the different siRNA we offer for in vivo studies:
Ambion In Vivo Pre-designed siRNAs are designed using the Silencer Select algorithm and incorporate additional chemical modifications for superior serum stability (half life >5 hours at 37 degrees C in 90% mouse serum) with in vivo applications. Ambion In Vivo siRNAs are non-toxic and non-immunogenic in vitro (peripheral blood nononuclear cells; PBMC) and in vivo (mouse). In cell-based assays, Ambion In Vivo siRNAs exhibit potency and specificity equivalent to Silencer Select siRNAs.
Ambion In Vivo Custom Designed siRNAs are designed to your specified target, using the Silencer Select algorithm and Ambion In Vivo chemical modifications.
Ambion In Vivo Custom Synthesized siRNAs are synthesized with your sequence, with Ambion In Vivo chemical modifications for serum stability.
Our Ambion In Vivo siRNA can be ordered with the following purities:
Desalted: Desalted and analyzed by MALDI-TOF mass spectrometry.
HPLC: HPLC purified and analyzed by MALDI-TOF and analytical HPLC
In Vivo Ready : HPLC purified, analyzed by MALDI-TOF and analytical HPLC, dialyzed to remove salts, sterile filtered, and endotoxin tested
Use our GeneAssist tool to order your custom siRNA (http://www.thermofisher.com/order/custom-genomic-products/tools/sirna/).
For Research Use Only. Not for use in diagnostic procedures.