Recombinant Protein;1MG;ActiveMax Human IL-6, Tag Free (IL6-H4218) is expressed from human 293 cells (HEK293). It contains AA Val 30 - Met 212 (Accession # NP_000591).;This protein carries no "tag". The protein has a calculated MW of 20.8 kDa. The protein migrates as 23-30 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.;Immobilized ActiveMax Human IL-6, Tag Free (Cat. No. IL6-H4218) at 5 g/mL (100 L/well) can bind Biotinylated Human IL-6 R alpha, Avitag,His Tag (Cat. No. CD6-H82E8) with a linear range of 4-125 ng/mL (QC tested).;ActiveMax Human IL-6, Tag Free (Cat. No. IL6-H4218) stimulates proliferation of TF-1 human erythroleukemic cell line. The EC50 for this effect is 0.2856-0.3636 ng/mL (Routinely tested).
