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Applied Biosystems™ 7500 Real Time PCR Systems Spectral Calibration Kit I
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Catalog No. 4349180
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Catalog No. 4349180 Supplier Applied Biosystems™ Supplier No. 4349180
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Description

This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR™ Green I, VIC™/JOE™, and NED™/TAMRA™/ROX™ dyes on the 7500 Real-Time PCR System.

The Applied Biosystems™ 7500 Real-Time PCR Systems Spectral Calibration Kit I contains one background plate, one region of interest (ROI) calibration plate, and seven spectral calibration plates with seven separate dye standards.

4349180

  • 96-well optical reaction plates preloaded with dyes (FAM™/SYBR™ Green I, VIC™/JOE™, NED™/TAMRA™/ROX™) are provided for quick and easy calibration of the 7500 Real-Time PCR System
  • Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture
  • Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions

4351151

  • 96-well optical reaction plates preloaded with Cy3™, Cy5™, and Texas Red™ dyes allow quick and easy calibration of the 7500 Real-Time PCR System
  • Easy-to-use, preloaded spectral calibration kit allows accurate discrimination of the individual dyes in a reaction mixture when all the dyes are excited simultaneously

Spend Your Time on Research, Not Instrument Calibration:
This kit is easy-to-use, preloaded, 96-well optical reaction plates enable users to calibrate the 7500 Real-Time PCR System quickly and easily. Insert the reaction plates during system installation, and the system automatically collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Content And Storage Includes one background plate, seven spectral calibration plates with FAM™/SYBR™ Green I, VIC™/JOE™, and NED™/TAMRA™/ROX™ dye standards, and one ROI calibration plate.

Store at -15°C to -25°C. Protect from light.
Calibrated Dye FAM, JOE, NED, ROX, TAMRA™, VIC, SYBR™ Green
For Use With (Equipment) 7500 System
Detection Method Fluorescence
Product Type Spectral Calibration Kit
For Use With (Application) Real Time PCR (qPCR)
Shipping Condition Dry Ice
Quantity 1 kit
Calibration Type Spectral Calibration

Frequently Asked Questions (FAQs)

What type of Real-Time PCR (Sequence Detection System) information is available from Applied Biosystems?

Tutorials demonstrating introductions to Real-Time PCR, guidelines for designing real-time assays using Primer Express software, and various instrument set-ups can be found under Life Technologies University on our website. User bulletins, manuals and Product inserts, protocols can be found as downloadable pdf files on our Technical Resources page.

For Research Use Only. Not for use in diagnostic procedures.