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7500 Fast System Upgrade Kit

Catalog No. 4362143
Encompass
Catalog No. 4362143 Supplier Thermo Fisher Scientific Supplier No. 4362143

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

The Applied Biosystems™ 7500 Fast Real-Time PCR System enables standard 96-well format high speed thermal cycling, significantly reducing your run time for quantitative real-time PCR applications, delivering results in about 30 minutes. The Upgrade Kit is available to upgrade a standard 7500 Real-Time PCR System to the Fast Configuration via a field service installation.

For Research Use Only. Not for use in diagnostics procedures.

Specifications

Content And Storage Package includes 7500 Fast Block replacement and supporting system updates. Recommended operating conditions; Temperature: 15 to 30 °C (59 to 86 °F). Maximum change of less than 15 degrees Celsius (27 °F) per 24 hrs. Humidity: 20 to 80% relative humidity, noncondensing.
For Use With (Equipment) 7500 Fast System, 7500 System
Product Type System Upgrade Kit
For Use With (Application) Real Time PCR (qPCR)
Quantity 1 kit

Frequently Asked Questions (FAQs)

What are the thermal cycling parameters of the Fast mode for the 7500 Fast Real-Time PCR System?

For the 7500 Fast real time PCR systems, the thermal cycling parameters are defaulted to 50°C/2 min for UNG incubation, 95°C/20 sec for fast polymerase activation, 40 cycles of 95°C/3 secs for the dissociation and 60°C/30 seconds for annealing and extensions.
Can I run slower, standard thermal cycling experiments on a 7500 Fast Real-Time PCR System?

Yes. you can choose either standard or fast ramp on the 7500 Fast System for software versions 2x or greater, and a 9600 emulation thermal cycling mode is available for customers who wish to run assays based on adjusted ramp rate. However, you should be aware that we cannot guarantee identical performance to the same modes run on a standard 7500 system block.
Can I compare the data collected when using Fast thermal cycling mode compared to using standard thermal cycling mode on the 7500 Fast Real-Time PCR System?

Runs can be compared between standard and fast mode, as the data collected are collected in all five filters in the instrument. However, keep in mind that these data may not always show identical results and may have added variability due to the data collection processes and differences in ramp rate between standard and fast mode.

If I were to upgrade my existing 7500 System standard unit to the a 7500 Fast configuration, can I still open my old data files?

Yes, older 7500 or 7500 fast data files are compatible with the current version of the SDS software. However, there is one exception: please note that the SDS v1.3 software will not allow RQ Studies to be created from RQ Plates from different instrument types, different thermal modes (i.e., fast and standard modes) or different numbers of thermal cycles.
Which computer operating systems are compatible with the7500 Fast Real-Time PCR System?

You can use up to Windows XP, Service Pack 3 (SP3) on the computer that is connected to the 7500 Fast Real-Time PCR System instrument. An exception is the SDS v2.0.5 software, which can be used with the Windows 7 operating system for stand-alone data analysis steps.

What is the recommended input template amount for each quantitative reaction on the 7500 Fast Real-Time PCR System?

The recommended input template range is 10-100 ng of gDNA/ cDNA template per reaction.
What is the recommended reaction volume that I can run for using 7500 Fast Real-Time PCR System?

We recommend running a total reaction volume between 10 and 30 ul per well.
To analyze an RQ Study, can I import RQ plates from a combination of Fast and Standard mode runs on a 7500 Fast Real-Time PCR System?

We do not recommend this as the current and previous versions of SDS software will not allow RQ Studies to be created from RQ Plates from different instrument types, different thermal modes/profiles or different numbers of thermal cycles. It is important to reduce variability among RQ plates in an RQ study by importing RQ plates from the same instrument type, with the same thermal profile and mode, as well as the same block type and sample volume.
Can I run "standard" and "9600 emulation" modes on the 7500 Fast Real-Time PCR System?

Yes, standard and 9600 emulation modes are optionally available. We have matched the ramping rate and thus anticipate equivalent performance. However, the actual total run times will differ from standard and 9600 emulation modes run on a standard block, and we cannot guarantee identical results as performance may vary depending on the assay.
Are there differences in the tube/plate format between Applied Biosystems 7500 Real-Time PCR Systems and Applied Biosystems 7500 Fast Real-Time PCR Systems?

Yes, the standard 7500 instrument has a 96 x 0.2 mL block, whereas the 7500 Fast instrument has a 96 x 0.1 mL block.

For Research Use Only. Not for use in diagnostic procedures.