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Applied Biosystems™ TaqMan™ RNase P Instrument Verification Plate for 7300/7500 Systems, 96-well

Catalog No. 4350584
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Used to verify the performance of the Applied Biosystems™ 7300 and 7500 RT-PCR Systems.

The 7300 and 7500 Real-Time PCR Systems TaqMan™ RNase P Instrument Verification Plate is a 96-well PCR plate preloaded with the necessary reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each well contains preloaded reaction mix (1X TaqMan™ Universal PCR Master Mix, RNase P primers, and FAM™ dye–labeled probe) and template. The 7300 and 7500 Real-Time PCR Systems TaqMan™ RNase P Instrument Verification Plate satisfies the same requirements as the Applied Biosystems™ 7300 and 7500 Real-Time PCR Systems installations. To pass installation, the instruments must demonstrate the ability to distinguish between 5,000 and 10,000 genomic equivalents with a 99.7% confidence level for a subsequent sample run in a single well. To ensure accurate results, Applied Biosystems™ recommends that the performance of your instrument be verified every six months.

Specifications

Content And Storage Contains one 96-well MicroAmp PCR tray pre-loaded and sealed with RNaseP TaqMan™ reagents, including probes and primers.

Store at -15°C to -25°C. Protect from light.
Calibrated Dye FAM
For Use With (Equipment) 7300 System, 7500 System
Detection Method Primer-probe
Product Line TaqMan
Product Type Rnase P Instrument Verification Plate
For Use With (Application) Real Time PCR (qPCR)
Shipping Condition Dry Ice
Quantity 1 kit
Calibration Type RNase P Calibration, Instrument Verification Plate
PCR Method qPCR
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Can I use my 7900HT System RNase P instrument verification plates with both the 7900HT System and the QuantStudio 6 and 7 Flex Real-Time PCR Systems?

Only the TaqMan RNase P Instrument Verification Plate, Fast 96-well (for 0.1 mL block) (Cat. No. 4351979) is compatible with both the 7900HT System and the QuantStudio 6 and 7 Flex Real-Time PCR Systems. The following RNase P instrument verification plates can be used on the 7900HT System, but not on the QuantStudio 6 and 7 Flex Real-Time PCR Systems:
• TaqMan RNase P Instrument Verification Plate, 96-well (Cat. No. 4310982) (for 96-well standard block)
• TaqMan RNase P Instrument Verification Plate for 7900HT System, 384-well (Cat. No. 4323306) (for 384-well block)
• 7900HT TaqMan Low Density Array Instrument Verification RNase P Kit (Cat. No. 4351468) (for TaqMan Array Card block)

The following RNase P instrument verification plates can be used on the QuantStudio 6 and 7 Flex Real-Time PCR Systems, but not on the 7900HT System:
• TaqMan RNase P Instrument Verification Plate for ViiA7 System, 96-well (Cat. No. 4432382) (for 96-well standard block)
• TaqMan RNase P Instrument Verification Plate for ViiA7 System, 384-well (Cat. No. 4455280) (for 384-well block)
• ViiA7 Array Card RNase P Verification Kit (Cat. No. 4432265) (for TaqMan Array Card block)

What type of Real-Time PCR (Sequence Detection System) information is available from Applied Biosystems?

Tutorials demonstrating introductions to Real-Time PCR, guidelines for designing real-time assays using Primer Express software, and various instrument set-ups can be found under Life Technologies University on our website. User bulletins, manuals and Product inserts, protocols can be found as downloadable pdf files on our Technical Resources page.

For Research Use Only. Not for use in diagnostic procedures.