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5-Bromo-3-indolyl beta-d-galactopyranoside, 98+%

Catalog No. AAJ65978MC
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AAJ6597803 1 g
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Catalog No. AAJ65978MC Supplier Thermo Scientific Chemicals Supplier No. J65978MC

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alpha-Galactosidase substrate for Lac-gene detection systems | CAS: 97753-82-7 | C14H16BrNO6 | 374.19 g/mol

5-Bromo-3-indolyl-β-D-galactopyranoside is an α-galactosidase substrate which is converted to an insoluble indigo-blue chromophore darker than that released by X-GAL. It is ideal for Lac gene detection systems in immunoblotting, immunocytochemical, and histological applications. Also used as chromogenic substrate that is suitable for identification of lac-(+) bacterial colonies.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
5-Bromo-3-indolyl-β-D-galactopyranoside is an α-galactosidase substrate which is converted to an insoluble indigo-blue chromophore darker than that released by X-GAL. It is ideal for Lac gene detection systems in immunoblotting, immunocytochemical, and histological applications. Also used as chromogenic substrate that is suitable for identification of lac-(+) bacterial colonies.

Solubility
Soluble in dimethylformamide (100 mg/ml), dimethylformamide/water (1:1 v/v) (10 mg/ml - clear, practically colorless solution).

Notes
Store in freezer (-20°C). Keep the container tightly closed. Store away from oxidizing agent.

Chemical Identifiers

CAS 97753-82-7
Molecular Formula C14H16BrNO6
Molecular Weight (g/mol) 374.19
MDL Number MFCD00063691
InChI Key LINMATFDVHBYOS-MBJXGIAVSA-N
Synonym 5-bromo-3-indolyl-beta-d-galactopyranoside, 5-bromo-3-indolyl-b-d-galactopyranoside, bluo-gal, blue-gal, 2s,3r,4s,5r,6r-2-5-bromo-1h-indol-3-yl oxy-6-hydroxymethyl oxane-3,4,5-triol, 5-brig, blue gal, 5-bromo-3-indolyl-beta-galactoside, 5-bromo-3-indolyl beta-d-galactopyranoside, 5-bromo-3-indoxyl-beta-d-galactopyranoside
PubChem CID 126898
IUPAC Name (2S,3R,4S,5R,6R)-2-[(5-bromo-1H-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol
SMILES OC[C@H]1O[C@@H](OC2=CNC3=CC=C(Br)C=C23)[C@H](O)[C@@H](O)[C@H]1O

Specifications

Color White
Quantity 100 mg
Solubility Information Soluble in dimethylformamide (100mg/ml),dimethylformamide/water (1:1 v/v) (10mg/ml- clear,practically colorless solution).
Formula Weight 374.18
Percent Purity ≥98%
Physical Form Powder
Chemical Name or Material 5-Bromo-3-indolyl beta-d-galactopyranoside
TSCA No
Recommended Storage Store at -20°C

RUO – Research Use Only