Get Up to $3,000 in Extra qPCR Products with Purchase
When you buy up to $3,000 of Applied Biosystems™ qPCR consumables and reagents,you’ll get a matching amount of qPCR products at no additional cost. After making your qualifying purchase, log in to the Redemption Center to redeem this offer.
Features and Benefits
Real-time PCR, also called quantitative or qPCR, is the gold-standard for detecting and quantifying nucleic acid targets. Applied Biosystems offers optimized master mixes, assay algorithms, intuitive data analysis software, and smart instrumentation give you the right tools for your applications. Their products are designed to work synergistically to help create a flexible and efficient qPCR workflow.
Qualifying Products

Applied Biosystems™ Power SYBR™ Green PCR Master Mix 
Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.
GC-Rich PCR Performance | High |
---|---|
Purity or Quality Grade | LD (Low DNA) |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Detection Method | SYBR |
Polymerase | AmpliTaq Gold DNA Polymerase, AmpliTaq Gold LD |
Product Type | Real Time PCR SYBR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7300 System, 7500 System, 7700 System, 7900HT Fast System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
Label or Dye | SYBR Green |
Product Line | SYBR™ |
Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR 
Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.
Shipping Condition | Wet Ice |
---|---|
Polymerase | Dual-Lock Taq DNA Polymerase |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, QuantStudio™ Absolute Q Digital PCR System, StepOne™, StepOnePlus™, ViiA™ 7 System |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Product Line | PowerUP™, SYBR™ |
Detection Method | SYBR |
Applied Biosystems™ TaqMan™ Fast Advanced Master Mix for qPCR 
TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms.
Shipping Condition | Wet Ice |
---|---|
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2 X |
Form | Liquid |
Concentration | 2X |
For Use With (Application) | Gene Expression,miRNA analysis,Virus Detection |
PCR Method | qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Fast DNA Polymerase |
Product Type | Fast Advanced Master Mix |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio Absolute Q Digital PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 6 Pro, QuantStudio™ 7 Flex, QuantStudio™ 7 Pro, StepOne™, StepOnePlus™, ViiA™ 7 System |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Fast |
Product Line | TaqMan™ |
Applied Biosystems™ TaqMan™ Universal PCR Master Mix, no AmpErase™ UNG 
TaqMan™ Universal PCR Master Mix, No AmpErase™ UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications.
Shipping Condition | Wet Ice |
---|---|
Polymerase | AmpliTaq Gold DNA Polymerase |
GC-Rich PCR Performance | High |
Product Type | Universal PCR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
PCR Method | qPCR |
Product Line | TaqMan™ |
Detection Method | Primer-probe |
Applied Biosystems™ TaqMan™ Multiplex Master Mix 
Applied Biosystems™ TaqMan™ Multiplex Master Mix is formulated to amplify up to 4 targets in a single reaction, delivering shorter run times (less than 40 minutes).
Polymerase | AmpliTaq Fast DNA Polymerase |
---|---|
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2 X |
Product Type | Multiplex Master Mix |
Sample Type | DNA |
Reaction Speed | Fast |
Concentration | 2X |
For Use With (Application) | Gene Expression,Virus Detection |
PCR Method | Multiplex qPCR |
Product Line | TaqMan™ |
Detection Method | Primer-probe |
Applied Biosystems™ TaqMan™ Universal Master Mix II, with UNG 
TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).
GC-Rich PCR Performance | High |
---|---|
Content And Storage | Store at 2°C to 8°C in the dark. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). |
Fidelity (vs. Taq) | 2 X |
Purity or Quality Grade | UP (Ultra Pure) |
Concentration | 2X |
For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
PCR Method | qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Gold DNA Polymerase |
Product Type | Universal Master Mix II |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
Product Line | TaqMan™ |
Invitrogen™ ROX Reference Dye 
ROX Reference Dye is designed to normalize the fluorescent reporter signal in real-time quantitative PCR or RT-PCR.
Content And Storage | Store in freezer (-5° to -30°C). |
---|---|
Label or Dye | ROX |
Detection Method | Primer-probe |
Applied Biosystems™ TaqMan™ Universal Master Mix II, no UNG 
TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).
GC-Rich PCR Performance | High |
---|---|
Content And Storage | Store at 2°C to 8°C in the dark. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). |
Fidelity (vs. Taq) | 2 X |
Purity or Quality Grade | UP (Ultra Pure) |
Concentration | 2X |
For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
PCR Method | qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Gold DNA Polymerase |
Product Type | Universal Master Mix II |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
Product Line | TaqMan™ |
Thermo Scientific™ Verso 1-step RT-qPCR Kit, SYBR Green, low ROX
The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.
GC-Rich PCR Performance | High |
---|---|
Format | Tube |
For Use With (Equipment) | 7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P |
Sample Type | RNA |
PCR Method | 1-step RT-qPCR |
Product Line | Verso |
Detection Method | SYBR |
Thermo Scientific™ ABsolute Blue QPCR Mix, SYBR Green, low ROX
Incorporates an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.
Polymerase | Taq DNA Polymerase |
---|---|
GC-Rich PCR Performance | High |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
For Use With (Equipment) | 7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P |
Sample Type | DNA, dsDNA |
Reaction Speed | Standard |
Concentration | 2X |
Compatibility | qPCR Cyclers |
For Use With (Application) | Gene Expression,Genotyping,siRNA Validation |
PCR Method | qPCR |
Product Line | ABsolute Blue |
Detection Method | SYBR |
Applied Biosystems™ TaqMan™ β-Actin Detection Reagents
TaqMan™ β-Actin Detection Reagents
Applied Biosystems™ TaqMan™ GAPDH Control Reagents (human) 
TaqMan™ GAPDH Control Reagents (human)
Applied Biosystems™ Human ACTB (Beta Actin) Endogenous Control (FAM™/MGB probe, non-primer limited) 
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More

Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More
Human ACTB (Beta Actin) Endogenous Control (FAM™/MGB probe, non-primer limited)
Shipping Condition | Room Temperature |
---|---|
Content And Storage | Control Primer-Probe Set(s) |
Form | Liquid |
Gene Symbol | ACTB |
Green Features | Sustainable packaging |
Amplicon Size | 171 |
Concentration | 20X |
For Use With (Application) | Gene Expression |
PCR Method | 2-step RT-qPCR |
Detection Method | Primer-probe |
Accession Number | NM_001101.2 |
Target | β-actin |
Format | Tube |
Label or Dye | FAM |
Species | Human |
Product Line | FAM™, TaqMan™ |
Applied Biosystems™ Human GUSB (Beta Glucuronidase) Endogenous Control (FAM™/MGB probe, non-primer limited) 
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More

Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More
Human GUSB (Beta Glucuronidase) Endogenous Control (FAM™/MGB probe, non-primer limited)
Shipping Condition | Room Temperature |
---|---|
Content And Storage | Control Primer-Probe Set(s) |
Gene Symbol | GUSB |
Green Features | Sustainable packaging |
Amplicon Size | 81 |
Concentration | 20X |
For Use With (Application) | Gene Expression |
PCR Method | 2-step RT-qPCR |
Detection Method | Primer-probe |
Accession Number | NM_000181.1 |
Target | GUSB |
Format | Tube |
Label or Dye | FAM |
Species | Human |
Product Line | FAM™, TaqMan™ |
Applied Biosystems™ Human GAPD (GAPDH) Endogenous Control (FAM™/MGB probe, non-primer limited) 
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More

Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More
Human GAPD (GAPDH) Endogenous Control (FAM™/MGB probe, non-primer limited)
Shipping Condition | Room Temperature |
---|---|
Content And Storage | Control Primer-Probe Set(s) |
Form | Liquid |
Gene Symbol | GAPDH |
Green Features | Sustainable packaging |
Amplicon Size | 122 |
Concentration | 20X |
For Use With (Application) | Gene Expression |
PCR Method | 2-step RT-qPCR |
Detection Method | Primer-probe |
Accession Number | NM_002046.3 |
Target | GAPDH |
Format | Tube |
Label or Dye | FAM |
Species | Human |
Product Line | FAM™, TaqMan™ |
Restrictions
Offer valid from July 1, 2023 to June 30, 2024. Redemptions must be received by June 30, 2025.
Redeemed product value is based on Fisher Scientific list price. The total value of redemption items cannot exceed the maximum redeemable threshold of the promotion. Offer can be redeemed one time per new laboratory. Offer cannot be combined with any other offers. Orders cannot be combined to qualify unless otherwise stated. Each qualifying item can only count towards one promotion redemption.
NOTE: Assay tools are not available in Canada.