MilliporeSigma Western Blotting Solutions
Designed to Help You Work More Efficiently
Get consistent and accurate results with a comprehensive range of MilliporeSigma products for western blotting:
- The SNAP I.d.™ System, a fast detection platform
- Immobilon™-P membrane, the most referenced PVDF membrane on the market
- Immobilon-E, the first prewet PVDF membrane without alcohol
- Plus detection reagents that are simple to use
Immobilon NOW Transfer Membrane Video
See how to get perfectly sized western blotting membrane every time with Immobilon NOW Transfer Membranes.
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Immobilon™-E Transfer Membrane
Available in a roll or easy-to-use precut sheets that fit standard mini or midi blots, these membranes eliminate the need for alcohol pre-wetting.
Immobilon-E Blotting Sandwiches
A convenient way to process multiple Western blots for standard tank transfers. Each sandwich has Immobilon-E membranes interleaved with sheets of blotting paper.
Immobilon-NOW Transfer Membranes
Compatible with mini or midi gels, these membranes offer the convenience of pre-cut sheets with the flexibility of membrane rolls.
Immobilon Transfer Membrane Half Rolls
Save money and custom-cut your Immobilon-E, -FL, -P, or ‑PSQ transfer membranes from these convenient half rolls.
Immobilon Signal Enhancer for Immunodetection
Combines signal amplification and blocking in a ready-to-use reagent that amplifies low signal intensity without amplifying the noise.
Immobilon Block Noise Canceling Reagents
Minimize background noise for easier detection of even low-abundance proteins with these protein-free blocking reagents.
ReBlot™ Antibody Stripping Solution
Now you can re-use your western blots ─ strip just the antibodies from blots at room temperature in approximately 15 minutes.
ImmobilonWestern Chemiluminescent HRP Substrate
Combining high sensitivity and low background, this substrate provides high sensitivity over a broad detection range.
ImmobilonECL Ultra Western HRP Substrate
This ultra-sensitive reagent has a long signal duration for detecting horseradish peroxidase (HRP)-labeled antibodies on Western blots.
SNAP i.d.™ 2.0 Protein Detection System
Uses a vacuum to actively drive antibodies and buffers right through the membrane to shorten the time for blocking, washing, and antibody incubations to just 30 minutes.
Immobilon-P PVDF Transfer Membranes
Optimized for binding proteins transferred from a variety of gel matrices with superior handling, staining, and resistance.