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Thermo Scientific™ Zeba™ Spin Desalting Plates

Perform high-throughput sample desalting for mass spectrometry, HPLC, capillary electrophoresis, metabolite screening and assay development.

$352.80 - $737.10

Specifications

Capacity (Metric) 100uL
Formulation Polypropylene high-profile 96-well filter plates containing resin
View More Specs
Products
Catalog Number Mfr. No. Description Quantity Price Quantity    

PI87774

 
thermo scientific™
87774
Zeba Spin Desalting Plates, 40K MWCO 2 plates Pack of 2 Each for $352.80

PI87775

 
thermo scientific™
87775
Zeba Spin Desalting Plates, 40K MWCO 4 plates Pack of 4 Each for $640.50

PI89807

 
thermo scientific™
89807
Zeba Spin Desalting Plates, 7K MWCO 2 plates Each for $407.40

PI89808

 
thermo scientific™
89808
Zeba Spin Desalting Plates, 7K MWCO 4 plates Pack of 4 Each for $737.10
Description & Specifications

Specifications

Capacity (Metric) 100uL
Formulation Polypropylene high-profile 96-well filter plates containing resin

Thermo Scientific™ Zeba Spin Desalting Plates provide high-throughput removal of salt and small molecules from samples, preparing them for mass spectrometry, HPLC, capillary electrophoresis, metabolite screening and assay development.

These 96-well filter plates provide high-throughput desalting and buffer exchange for 20 to 100μL samples. Each well contains high-performance size-exclusion chromatography resin (Zeba Desalting Resin) that effectively traps and removes salts and small molecules upon addition of the sample(s) and centrifugation of the plate assembly. The desalted samples are recovered in a standard 96-well collection plate, making them ready for downstream handling and processing using multichannel pipetter or automated liquid handlers. The Zeba 96-well Spin Desalting Plates require no resin dispensing or hydration. One plate of 96 samples can be processed in five minutes.

Zeba Desalting Resin provides exceptional desalting and protein recovery characteristics. For the designated sample volumes (20 to 100μL), these plates provide exceptional protein recovery and at least 95% removal of salts and other small molecules. Proteins that are significantly larger than the molecular-weight cutoff (MWCO) rating of the resin are recovered at greater than 95%; proteins that are only slightly larger than the MWCO rating are recovered at approximately 85%. Desalting plates with 7K and 40K MWCO resins are offered.

Desalting is a critical step in identifying and quantifying proteins in biological systems, and sample prep automation is a desirable goal for enabling high throughput proteomics analysis. These ready-to-use plates meet this goal, enabling rapid sample prep for an array of downstream analyses such as mass spectrometry, HPLC, capillary electrophoresis, metabolite screening, and assay development.

Highlights:

  • High throughput – 96-well centrifuge filter plate format; desalt 96 samples in 5 minutes

  • Ready to use – no resin dispensing or plate preparation required; wash and collection plates included

  • Downstream compatible – desalted samples are collected in 96-well plates, ready for downstream handling and processing using multichannel pipetter or automated liquid handlers

  • Replaces drip-column methods – no need to collect and screen slowly emerging protein fractions

  • Exceptional protein yields – recover greater than 95% of the protein with minimal dilution in typical applications

  • 7K and 40K MWCO options – choose plates with 7K MWCO resin to recover all proteins larger than 7000 daltons with high efficiency; choose plates with 40K MWCO resin to selectively recover only large proteins with high efficiency

Includes:

Desalting filter plates (pre-filled with Zeba Desalting Resin) and an equal number of wash plates and collection plates

Requires:

Variable-speed centrifuge with rotor and carrier capable of handling stacked plates (height = 4.4cm) at 1000 x g