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Thermo Scientific™ Verso 1-step RT-qPCR SYBR Green Kits

Perform 1-step RT-qPCR using SYBR Green chemistry with these ready-to-use optimized solutions.

$776.29 - $776.29

Specifications

For Use With (Application) Ideal for a range of applications including gene expression analysis, siRNA knockdown validation, miRNA detection, microarray validation, pathogen detection and disease research
Includes Verso Enzyme Mix (RNase inhibitor, RT Enhancer, Thermo-Start™ Taq DNA Polymerase‡, High performance dNTPS, SYBR Green I, ROX™ passive reference dye (only in ROX mixes), Fluorescein (only in Fluorescein mix)); Separate MgCl2 vial for further MgCl2 optimization; Separate ROX vial (only in kits indicated)
Storage Requirements Store at -20°C. Avoid freeze/thaw cycles. Protect from light
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 Disclaimers

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Products
Catalog Number Mfr. No. Description Quantity Price Quantity    

AB4104A

 
thermo scientific™
AB-4104/A
With separate ROX vial 200 x 25µL Rxns CALL N/A N/A
 Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

AB4105A

 
thermo scientific™
AB-4105/A
With ROX 200 x 25µL Rxns Pack of 2 Each for $776.29

AB4106A

 
thermo scientific™
AB-4106/A
With Low ROX 200 x 25µL Rxns Pack of 2 Each for $776.29
Description & Specifications

Specifications

For Use With (Application) Ideal for a range of applications including gene expression analysis, siRNA knockdown validation, miRNA detection, microarray validation, pathogen detection and disease research
Includes Verso Enzyme Mix (RNase inhibitor, RT Enhancer, Thermo-Start™ Taq DNA Polymerase‡, High performance dNTPS, SYBR Green I, ROX™ passive reference dye (only in ROX mixes), Fluorescein (only in Fluorescein mix)); Separate MgCl2 vial for further MgCl2 optimization; Separate ROX vial (only in kits indicated)
Storage Requirements Store at -20°C. Avoid freeze/thaw cycles. Protect from light

Verso 1-step RT-qPCR Kits for SYBR Green chemistry offer a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for Dnase I treatment. All Verso 1-step RT-qPCR kits incorporate an inert dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

Highlights

  • Eliminate the need for DNase treatments - RT Enhancer (optional) prevents gDNA carry-over
  • RT and qPCR steps performed in single tube - convenience and reduced contamination risk
  • Use of the entire RT reaction as template for qPCR - improved sensitivity
  • Verso RT enzyme is highly sensitive with broad dynamic range - accurate detection of RNA from 1 pg-1 ug
  • Verso RT enzyme has a wide working temperature range (42 to 57°C) - improved success with GC-rich and other difficult templates
  • Contains an inert blue dye for ease of pipetting during reaction setup

In a 1-step approach to RT-qPCR, both the RT step and qPCR steps are performed in a single tube (1-step RT-qPCR). Adopting a 1-step approach is ideal for primer sequence optimization and collecting comparative data in experiments that do not require subsequent cDNA storage. The approach minimizes the risk of contamination, and improvements in reproducibility may be observed as a result of reduced sample handling.

Verso 1-Step RT-qPCR Kits for SYBR Green chemistry are compatible with all major real-time thermal cyclers.