Please login to your online account to display your discounted pricing

Thermo Scientific™ Verso™ 1-Step RT-qPCR Kits

Perform 1-step RT-qPCR using probe chemistry with these ready-to-use, optimized solutions.

$593.82 - $1,072.92

Specifications

Includes Verso Enzyme Mix (RNase inhibitor, RT Enhancer, Thermo-Start™ Taq DNA Polymerase1, High performance dNTPS, ROX™ passive reference dye (only in ROX mixes); Separate ROX vial (only in kits indicated)
Storage Requirements Store at -20°C. Avoid freeze/thaw cycles
View More Specs

 Disclaimers

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Products
Catalog Number Mfr. No. Product Type Quantity Price Quantity    

AB4100C

 
thermo scientific™
AB-4100/C
1-Step QRT-PCR Kit plus separate ROX vial 400 x 25µL Rxns Each for $1,072.92

AB4101A

 
thermo scientific™
AB-4101/A
1-Step QRT-PCR Kit w/ROX 200 x 25µL Rxns Pack of 2 Each for $593.82

AB4101C

 
thermo scientific™
AB-4101/C
1-Step QRT-PCR Kit w/ROX 400 x 25µL Rxns Each for $1,072.92

AB4102A

 
thermo scientific™
AB-4102/A
1-Step QRT-PCR Kit w/low ROX 200 x 25µL Rxns Pack of 2 Each for $593.82
Description & Specifications

Specifications

Includes Verso Enzyme Mix (RNase inhibitor, RT Enhancer, Thermo-Start™ Taq DNA Polymerase1, High performance dNTPS, ROX™ passive reference dye (only in ROX mixes); Separate ROX vial (only in kits indicated)
Storage Requirements Store at -20°C. Avoid freeze/thaw cycles

Verso 1-step RT-qPCR Kits for probe chemistry offer a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for Dnase I treatment. All Verso 1-step RT-qPCR kits incorporate an inert dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

Highlights

  • Eliminate the need for DNase treatments -RT Enhancer (optional) prevents gDNA carry-over
  • RT and qPCR steps performed in single tube -convenience and reduced contamination risk
  • Use of the entire RT reaction as template for qPCR -improved sensitivity
  • Verso RT enzyme is highly sensitive with broad dynamic range -accurate detection of RNA from 1 pg-1 ug
  • Verso RT enzyme has a wide working temperature range (42 to 57°C) -improved success with GC-rich and other difficult templates
  • Contains an inert blue dye for ease of pipetting during reaction set up

In a 1-step approach to RT-qPCR, both the RT step and qPCR steps are performed in a single tube (1-step RT-qPCR). Adopting a 1-step approach is ideal when no cDNA storage is necessary for subsequent experiments. The approach minimizes the risk of contamination and improves reproducibility by reducing sample handling.

Verso 1-step RT-qPCR kits for probe chemistry are compatible with all standard probe chemistries, including Solaris qPCR Gene Expression Assays. They are also compatible with all major real-time thermal cyclers.