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UTF1 Mouse anti-Mouse, Clone: MFCDA84, eBioscience™

Mouse Monoclonal Antibody

$143.55 - $346.55

Specifications

Antigen UTF1
Clone MFCDA84
Host Species Mouse
Gene Alias Undifferentiated embryonic cell transcription factor
Species Reactivity Mouse
View More Specs

 Disclaimers

For Research Use Only.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

501122884

 
affymetrix
14984980
25μg Each for $143.55

501122885

 
affymetrix
14984982
100μg Each for $346.55
Description & Specifications

Specifications

Antigen UTF1
Clone MFCDA84
Host Species Mouse
Gene Alias Undifferentiated embryonic cell transcription factor
Species Reactivity Mouse
Applications Enzyme Linked Immunosorbent Assay
Applications Immunocytochemistry
Applications Immunohistochemistry (Formalin/Paraffin)
Applications Western Blotting
Regulatory Status RUO
Conjugate Unlabeled
Format Purified
Storage Requirements Store at 2-8°C.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Concentration 0.5mg/mL

The monoclonal antibody MFCDA84 recognizes mouse and human Undifferentiated embryonic cell Transcription Factor 1 (UTF1) which was first identified in mouse embryonic stem cells (inner cell mass and the epiblast) but is also expressed in human embryonic and adult stem cells. UTF1 transcription ceases at the onset of differentiation, which distinguishes it from less-sensitive pluripotency markers, such as Oct3/4 or Nanog. Importantly, its transcriptional regulation is very sensitive to differentiation cues and is switched off faster and with a larger magnitude than either Oct3/4 or Sox2 during embryoid body formation from both mouse and human ESCs. Reduction in UTF1 expression is associated with differentiation, although it does not appear to play a direct role in self renewal. UTF1 expression is regulated by Oct3/4 and is associated with chromatin. It is a transcriptional co-activator of ATF2 and is thought to play a role in maintaining an epigenetic environment necessary for maintained pluripotency. Co-expression of UTF1 with the reprogramming factors c-Myc, Oct3/4, Sox2, and KLF4, as well as reduction of p53 increases efficiency of iPS cell generation by 100 fold. In addition to its expression in stem cells, UTF1 is expressed in neuroblastoma and the presence of UTF1 along with BMI1 can distinguish low grade neuroblastomas from stage IV tumors.